Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Reexamination Certificate
2011-01-18
2011-01-18
Rao, Manjunath N (Department: 1656)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
C435S069100, C435S071100, C530S402000
Reexamination Certificate
active
07871794
ABSTRACT:
Compositions and methods are provided for the enhanced in vitro synthesis of active polypeptides containing disulfide bonds. In certain embodiments of the invention, the reaction mix includes a biological extract derived from a bacterial cell in which the glutathione reductase gene has been inactivated, which is pre-treated with a low concentration of a sulfhydryl inactivating agent.
REFERENCES:
patent: 6548276 (2003-04-01), Swartz et al.
patent: 7041479 (2006-05-01), Swartz et al.
patent: 2004/0038332 (2004-02-01), Swartz et al.
Bessette et al., “Efficient folding of proteins with multiple disulfide bonds in theEscherichia colicytoplasm,” Proc. Natl. Acad. Sci. USA, 1999, 96(24):13703-13708.
Bukau et al., “The Hsp70 and Hsp60 chaperone machines,” Cell, 1998, 92(3):351-366.
Calhoun et al., “Energizing cell-free protein synthesis with glucose metabolism,” Biotechnol. Bioeng., 2005, 90 (5):606-613.
Kim et al., “Efficient production of a bioactive, multiple disulfide-bonded protein using modified extracts ofEscherichia coli,” Biotechnol. Bioeng., 2004, 85(2):122-129.
Kim et al., “A highly efficient cell-free protein synthesis system fromEscherichia coli,” Eur. J. Biochem., 1996, 239 (3):881-886.
Kim et al., “Prolonging Cell-Free Protein Synthesis by Selective Reagent Additions,” Biotechnol. Prog., 2000, 16 (3):385-90.
Kim et al., “Prolonging Cell-Free Protein Synthesis with a Novel ATP Regeneration System,” Biotechnol. Bioeng., 1999, 66(3):180-188.
Missiakaset al., “Protein Folding in the Bacterial Periplasm, Minireview,” J Bacteriol., 1997, 179(8):2465-2471.
Noren et al., “A General Method for Site-Specific Incorporation of Unnatural Amino Acids into Proteins,” Science, 1989, 244(4901):182-188.
Qiu et al., “Expression of Active Human Tissue-Type Plasminogen Activator inEscherichia coli,” Appl. Environ. Microbiol., 1998, 64(12):4891-4896.
Richardson et al., “The ins and outs of a molecular chaperone machine,” Trends Biochem. Sci., 1998, 23(4):138-143.
Ryabova, “Functional antibody production using cell-free translation: Effects of protein disulfide isomerase and chaperones,” Nature Biotechnol., 1997, 15(1):79-84.
Voloshin et al., “Efficient and scalable method for scaling up cell free protein synthesis in batch mode,” Biotechnol. Bioeng., 2005, 91(4):516-521.
Knapp Kurtis G.
Swarts James Robert
Bozicevic Field & Francis LLP
Gebreyesus Kagnew H
Rao Manjunath N
Sherwood Pamela J.
The Board of Trustees of the Leland Stanford Junior University
LandOfFree
Enhanced cell-free synthesis of active proteins containing... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Enhanced cell-free synthesis of active proteins containing..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Enhanced cell-free synthesis of active proteins containing... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2717033