Engineering plant thioesterases and disclosure of plant thioeste

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

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435440, 536 236, C12N 1529, C12N 1552

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active

061505129

ABSTRACT:
Methods of altering substrate specificity of plant acyl-ACP thioesterases, and engineered plant acyl-ACP thioesterases so produced are provided. The C-terminal two thirds portion of plant thioesterases is identified as desirable for such modifications.
DNA sequences and constructs for expression of engineered thioesterases, as well as the novel thioesterases produced therefrom are also provided. Such DNA sequences may be used for expression of the engineered thioesterases in host cells, particularly seed cells of oilseed crop plants, for the modification of fatty acid composition. Of particular interest is a mangosteen Garm FatA1 18:1 thioesterase in which the relative 18:0 activity has been increased. Such FatA thioesterases find use for improved production of stearate in vegetable seed oils.

REFERENCES:
Yuan, L. "The Catalytic Cysteine and Histidine in the Plant Acyl-Acyl Carrier Protein Thioesterases" The Journal of Biological Chemistry 1996 PP. 3417-3419.
Yuan, L. "Modification of the substrate specificity of an acyl-acyl carrier protein thioesterase by protein engineering" Proc. Natl. Acad. Sci. USA col. 92 pp. 10639-10643. Nov. 1995.
Ohlrogge JB. Design of new plant products: Engineering of fatty acid metabolism. Plant Physiol. 104: 821-826, 1994.
Blundell TL. Problems and solutions in protein engineering--Towards rational design. Trends Biotech. 12: 145-148, 1994.

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