Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease
Reexamination Certificate
2005-09-27
2005-09-27
Rao, Manjunath N. (Department: 1652)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Transferase other than ribonuclease
C435S004000, C435S006120, C435S069100, C435S183000, C435S252300, C435S320100, C536S023200, C536S023400, C536S023500, C536S023700, C530S350000
Reexamination Certificate
active
06949372
ABSTRACT:
Methods for manipulating carbohydrate processing pathways in cells of interest are provided. Methods are directed at manipulating multiple pathways involved with the sialylation reaction by using recombinant DNA technology and substrate feeding approaches to enable the production of sialylated glycoproteins in cells of interest. These carbohydrate engineering efforts encompass the implementation of new carbohydrate bioassays, the examination of a selection of insect cell lines and the use of bioinformatics to identify gene sequences for critical processing enzymes. The compositions comprise cells of interest producing sialylated glycoproteins. The methods and compositions are useful for heterologous expression of glycoproteins.
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Betenbaugh Michael J.
Coleman Timothy A.
Lawrence Shawn
Lee Yuan C.
Human Genome Sciences Inc.
Rao Manjunath N.
The Johns Hopkins University
Whitham Curtis & Christofferson, PC
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