Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2007-12-11
2007-12-11
Strzelecka, Teresa E. (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091200, C536S023100, C536S024300
Reexamination Certificate
active
10251085
ABSTRACT:
Methods of amplifying nucleic acid have now been discovered which include the steps of: a) annealing a primer to a template nucleic acid sequence, the primer having a first portion which anneals to the template and a second portion of predetermined sequence; b) synthesizing a polynucleotide that anneals to and is complementary to the portion of the template between the location at which the first portion of the primer anneals to the template and the end of the template, the polynucleotide having a first end and a second end, wherein the first end incorporates the primer; c) separating the polynucleotide synthesized in step (b) from the template; d) annealing a nested oligonucleotide to the second end of the polynucleotide synthesized in step (b), the nested oligonucleotide having a first portion that anneals to the second end of the polynucleotide and a second portion having the same predetermined sequence as the second portion of the primer; e) extending the polynucleotide synthesized in step (b) to provide a terminal portion thereof that is complementary to the predetermined sequence; and f) amplifying the extended polynucleotide using a single primer having the predetermined sequence.
REFERENCES:
patent: 4582788 (1986-04-01), Erlich
patent: 4683194 (1987-07-01), Saiki et al.
patent: 5130238 (1992-07-01), Malek et al.
patent: 5508178 (1996-04-01), Rose et al.
patent: 5595891 (1997-01-01), Rose et al.
patent: 5658727 (1997-08-01), Barbas et al.
patent: 5679512 (1997-10-01), Laney et al.
patent: 5683879 (1997-11-01), Laney et al.
patent: 5744308 (1998-04-01), Guillou-Bonnici et al.
patent: 5962272 (1999-10-01), Chenchik et al.
patent: 6251639 (2001-06-01), Kurn
patent: 6291158 (2001-09-01), Winter et al.
patent: 6291159 (2001-09-01), Winter et al.
patent: 6291160 (2001-09-01), Lerner et al.
patent: 6291161 (2001-09-01), Lerner et al.
patent: 6403319 (2002-06-01), Lizardi et al.
patent: 6919189 (2005-07-01), Bowdish et al.
patent: 0050424 (1985-09-01), None
patent: 0084796 (1990-05-01), None
patent: 0237362 (1992-03-01), None
patent: 0201184 (1992-12-01), None
patent: 0258017 (1997-06-01), None
patent: WO 96/41012 (1996-12-01), None
patent: 9914226 (1999-03-01), None
patent: WO 00/08208 (2000-02-01), None
patent: WO 00/66779 (2000-11-01), None
patent: WO 01/06004 (2001-01-01), None
patent: WO 01/55454 (2001-08-01), None
Orum, H. et al., “Efficient method for constructing comprehensive murine Fab antibody libraries displayed on phage”, Nucl. Acids Res., vol. 21, pp. 4491-4498 (1993).
Zheleznaya, L. A. et al., “PCR Fragmentation of DNA”, Biochemistry (Moscow), vol. 64, pp. 373-378 (1999).
Mullis et al., Specific Enzymatic Amplification of DNA In Vitro: The Polymerase Chain Reaction, Cold Spring Harbor Symposia on Quantitative Biology, vol. LI, pp. 263-273, 1986.
Redding, Homologous Pairing and Strand Exchange in Genetic Recombination, Ann. Rev. Genet., 1982, 16:405-37.
Patel et al., Formation of Chimeric Dna Primer Extension Products by Template Switching onto an Annealed Downstream Oligonucleotide, Proc. Natl. Acad. Sci., USA, vol. 93, pp. 2969-2974, Apr. 1996.
Kuhn et al., DNA Helicases, Cold Spring Harbor Laboratory, 1978, pp. 63-67.
Ayala et al., “New Primer Strategy Improves Precision of Differential Display,”Biotechniques, 18(5): 842-844, 846, 848 and 850 (1995).
Cheung and Nelson, “Whole Genome Amplification Using a Degenerate Oligonucleotide Primer Allows Hundreds of Genotypes to be Performed on Less than One Nanogram of Genomic DNA,”Proceedings of the National Academy of Sciences of USA, 93:14676-14679 (1996).
Froussard, “A Random-PCR Method (rPCR) Construct Whole cDNA Library from Low Amounts of RNA,”Nucleic Acids Research, 20(11):2900 (1992).
Little et al., “Generation of a Large Complex Antibody Library from Multiple Donors,”Journal of Immunological Methods, 231(1-2):3-9 (1999).
Roux and Dhanarajan, “A Strategy for Single Site PCR Amplification of dsDNA: Priming Digested Cloned or Genomic DNA from an Anchor-Modified Restriction Site and a Short Internal Sequence,”Biotechniques, 8(1):48-57 (1990).
Tetenius et al., “Degenerate Oligonucleotide-Primed PCR: General Amplification of Target DNA by a Single Degenerate Primer,”Genomics, 13:718-725 (1992).
Moreno de Alboran, et al., “Human Monoclonal Fab Fragments Specific for Viral Antigens from Combinatorial IgA Libraries,”Immunotechnology, 1:21-28 (1995).
Bowdish Katherine S.
Frederickson Shana
Lin Ying-Chi
Maruyama Toshiaki
Renshaw Mark
Alexion Pharmaceuticals, Inc.
Ropes & Gray LLP
Strzelecka Teresa E.
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