Engineered secreted alkaline phosphatase (SEAP) reporter...

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S252300

Reexamination Certificate

active

10155229

ABSTRACT:
The invention provides a novel reporter gene (mSEAP) capable of being expressed in a mammal for extended periods of time. Nucleic acids encoding the reporter gene, cells and vectors comprising the nucleic acids, and methods of using the reporter gene to identify expression vectors and screen for drug compounds in an animal are also disclosed.

REFERENCES:
Mullins et al. (1996) J. Clin. Invest. vol. 98, pp. 1557-1560.
Mullins et al. (1993) Hypertension vol. 22, pp. 630-633.
Cameron (1997) Molec. Biotech. vol. 7, pp. 253-265.
Kappel et al. (1992) Current Opinion in Biotechnology, vol. 3, pp. 548-553.
Berger, et al., Secreted placental alkaline phosphatase: a powerful new quantitative indicator of gene expression in eukaryotic cells, Gene 66, 1-10 (1988).
Nilsson, et al., An in vivo mouse reporter gene (human secreted alkaline phosphatase) model to monitor ovarian tumor growth and rsponse to therapeutics, Cancer Chemotherapy and Pharmacology 49, 93-100 (2001).
Mir, et al., High-efficiency gene transfer into skeletal muscle mediated by electric pulses, PNAS 96, 4262-7, (1999).
Urlinger, et al., Exploring the sequence space for tetracycline-dependent transcriptional activators: Novel mutations yield expanded range and sensitivity, PNAS 97, 7963-68 (2000).
Manes, et al., Genomic Structure and Comparison of Mouse Tissue-Specific Alkaline Phosphatase Genes, Genomics 8, 541-554 (1990).
Hahnel, et al., Two alkaline phosphatase genes are expressed during early development in the mouse embryo, Development 110, 555-564 (1990).
Bao, et al., Use of a Surrogate Marker (Human Secreted Alkaline Phosphatase) to Monitor in Vivo Tumor Growth and Anticancer Drug Efficacy in Ovarian Cancer Xenografts, Gynecologic Oncology 78, 373-379 (2000).
Narisawa, et al., Embryonic alkaline phosphatase is expressed at M-phase in the spermatogenic lineage of the mouse, Development 116, 159-165 (1992).
Soubrier, et al., pCOR: a new design of plasmid vectors for nonviral gene therapy, Gene Therapy 6, 1482-1488 (1999).
Burbridge, et al., Biological and pharmacological characterisation of three models of human ovarian carcinoma established in nude mice: Use of the CA125 tumour marker to predict antitumour activity, International Journal of Oncology 15, 1155-1162 (1999).
Abruzzese, et al., Ligand-Dependent Regulation of Plasmid-Based Transgene Expression in Vivo, Human Gene Therapy 10, 1499-1507 (1999).
Mujtaba, et al., Stable Expression of the Alkaline Phosphatase Marker Gene by Neural Cells is Culture and after Transplanataion into the CNS Using Cells Derived from a Transgenic Rat, Experimental Neurology 174, 48-57 (2002).
Bettan, et al., High-Level Protein Secretion into Blood Circulation after Electric Pulse-Mediated Gene Transfer into Skeletal Muscle, Molecular Therapy 2, 204-210 (2000).
Latta-Mahieu, et al., Immunogenicity of the Tetracycline-Dependent Transcription Factor in Monkeys and its Impact on Reporter Gene Expression, Molecular Therapy 3, S398 (2001) (Abstract).
Rubinchik, et al., A Novel Adenovirus Vector Incorporating a Complex Tet Regulation System Delivers Tightly Regulated Transgene Expression With a Range of Over Three Orders of Magniture, Molecular Therapy 3, S398 (2001) (Abstract).
Excerpt from Applied Biosystems, Phospha-Light Secreted Alkaline Phosphatase Reporter Gene Assay System (2002).
Excerpt from Turner BioSystems Applications Note, A TD-20/20 Luminometer method for Secreted Embryonic Alkaline Phosphatase (SEAP), (Mar. 20, 2002).
Excerpt from Great EscAPe SEAP User Manual, CLONTECH Laboratories, Inc., Protocol #PT3057-1, Version #PR98443.
Excerpt from CLONTECHniques, Reporter Systems & Detection Kits, Oct. 2001.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Engineered secreted alkaline phosphatase (SEAP) reporter... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Engineered secreted alkaline phosphatase (SEAP) reporter..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Engineered secreted alkaline phosphatase (SEAP) reporter... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3809414

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.