Engineered cells producing insulin in response to glucose

Details Engineered cells producing insulin in response to glucose Engineered cells producing insulin in response to glucose

1992-01-13

1995-06-27

Hill, Jr., Robert J.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

435 4, 435 6, 435 691, 4351721, 4351722, 4351723, 4353201, 424520, 530350, 530397, 530303, 5303892, C12N 500, C12N 1500, C12Q 168, C12P 2106

Patent

active

054279402

ABSTRACT:
The present disclosure relates to the application of genetic engineering to provide artificial .beta. cells, i.e. cells which can secrete insulin in response to glucose. This is achieved preferably through the introduction of one or more genes selected from the insulin gene, glucokinase gene, and glucose transporter gene, so as to provide an engineered cell having all three of these genes in a biologically functional and responsive configuration. Assays for detecting the presence of diabetes-associated antibodies in biological samples using these and other engineered cells expressing diabetes-associated epitopes are described. Also disclosed are methods for the large-scale production of insulin by perfusing artificial .beta. cells, grown in liquid culture, with glucose-containing buffers.

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