Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1995-06-07
1999-12-07
Horlick, Kenneth R.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
536 243, C12Q 168, C07H 2104
Patent
active
059981350
ABSTRACT:
Disclosed is a nucleic acid hybridization assay composition for detecting the presence of absence of a target oligo- or polynucleotide in a sample. The composition comprises: a solid matrix having at least one surface which is substituted with a first intercalator capable of binding dsDNA dsRNA, or DAN-RNA hybrids; a second intercalator, which may or may not comprise at least one fluorophore, said intercalator or said fluorophore each acting as either an energy donor or an energy acceptor; and an oligo- or polynucleotide probe which is specifically hybridizable with the target oligo- or polynucleotide and has directly or indirectly bound thereto, at least one lanthanide metal chelate or at least one fluorophore, each acting as either an energy donor or an energy acceptor. Also disclosed are a method and kit for its use.
REFERENCES:
patent: 4257774 (1981-03-01), Richardson et al.
patent: 4547569 (1985-10-01), Letsinger et al.
patent: 4563417 (1986-01-01), Albarella et al.
patent: 4582789 (1986-04-01), Sheldon, III et al.
patent: 4637988 (1987-01-01), Hinshaw et al.
patent: 4670572 (1987-06-01), Hinshaw et al.
patent: 4707352 (1987-11-01), Stavrianopoulos
patent: 4707440 (1987-11-01), Stavrianopoulos
patent: 4711955 (1987-12-01), Ward et al.
patent: 4868103 (1989-09-01), Stavrianopoulos et al.
patent: 4921805 (1990-05-01), Gebeyehu et al.
Hemmilia, "Fluoroimmunoassays and Immunofluorometric Assays", Clin. Chem. 31(3):359-380 (1985).
Gibson P.E., et al., "Detection of Human Polyomavirus DNA in Urine Specimens by Hybridot Assay," Arch. of Virol. 84:233-240 (1985).
Soini, E. and Kojola, H., "Time-Resolved Fluorometer for Lanthanide Chelates, A New Generation of Nonisotopic Immunoassays, "Clin. Chem. 29(1):65-68 (1983).
Chan et al., Abst. of the Fifth Intl. Symposium on Rapid Methods & Automation in Microbiology and Immunology, Florence, Nov. 1987, p. 153.
Syvanen et al., "Time-resolved fluorometry: a sensitive method to quantify DNA-hybrids, "Nucleic Acids Research 14(2): 1017-1028 (1986).
Dahlen et al., "Sensitive detection of genes by sandwich hybridization and time-resolved fluorometry, "Molecular & Cellular Probes 1: 159-168 (1987).
Kubota et al., "Fluorescence Decay And Quantum Yield Characteristics of Acridine Orange And Proflavine Bound to DNA, "Biophysical Chemistry6:279-289 (1977).
Genest et al., "Investigation of DNA dynamics and drug-DNA interaction by steady state fluorescence anisotropy, "Nucleic Acid Research13:2603-2615 (1985).
Asseline et al., "Oligodeoxynucleotides covalently linked to intercalating dyes as base sequence-specific ligands. Influence of dye attachment site, "The EMBO Journal. 3(4):795-800 (1984).
Wakelin & Waring, , "The Unwinding of circular Deozyribonucleic Acid by Phenanthurinium Drugs: Structure-Activity Relations for the Intercalation Reaction", Mol. Pharm.9:544-561 (1974).
Wakelin & Waring, "Kinetics of Drug-DNA Interaction, "J. Mol. Biol. 144:183-214 (1980).
Saenger W., Principles of Nucleic Acid Structure, pp. 116-58 Springer-Verlag, New York.
Syvanen, "Nucleic Acid Hybridization: From Research Tool To Routine Diagnostic Method, "Medical Biology 64:313-324 (1986).
Syvanen et al., "Fast quantification of nucleic acid hybrids by affinity-based hybrid collection, "Nucleic Acids Research 14(12):5037-5048 (1986).
Sommer, R. and Tautz, D., "Minimal homology requirements for PCR primers, "Nucleic Acids Research 17(16):6749 (1989).
Georghiou, "Interaction of Acridine Drugs With DNA And Nucleotides," Photochemistry and Photobiology 26:59-68, Pergamon Press, Great Britain (1977).
Oser A. et al., "Sensitive non-radioactive dot-blot hybridization using DNA probes labelled with chelate group substituted psoralen and quantitative detection by europium ion fluorescence," Nuc. Acid Research 16(3):1181-1197 (1988).
Horrocks, W.D., et al., "Laser-Induced Lanthanide Ion Luminescence Lifetime Measurements by Direct Excitation of Metal Ion Levels, A New Class of Structural Probe for Calcium-Binding Proteins and Nucleic Acids," Journal of the American Chem. Society 99(7):2378-2380 (1977).
Chun, P.K., et al., International Symposium on Rapid Methods and Automation in Microbiology and Immunology (5.sup.th, 1987, Florence, Italy) published in Rapid Methods and Automation in Microbiology and Immunology, Balows, A., et al., Eds., Brixia Academic Press Brescia, pp. 572-577 (1989).
Saeger, W., Principles of Nucleic Acid Structure, Springer-Verlag, New York, 1984, pp. 116-158.
Nucleic Acids Research, vol. 17, No. 16, Sommer and Tautz, "Minimal Requirements for PCR primers", p. 6749, 1989.
Hurley Ian
Rabbani Elazar
Enzo Diagnostics, Inc.
Fedus, Esq. Ronald C.
Horlick Kenneth R.
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