Ends-out gene targeting method

Details Ends-out gene targeting method Ends-out gene targeting method

2007-10-23

2007-10-23

Crouch, Deborah (Department: 1632)

Multicellular living organisms and unmodified parts thereof and

Method of making a transgenic nonhuman animal

C800S022000

Reexamination Certificate

active

10434668

ABSTRACT:
A gene targeting method for use in a host organism whereby the host organism is transfected with an ends-out donor construct. Further transfecting the host organism with two transgenes expressing endonuclease and recombinase enzymes. The endonuclease and recombinase enzymes are used so that homologous recombination occurs between the DNA segment of the donor construct and a selected gene of the host organism, thus forming a host with containing the recombinogenic donor. The progeny of the host organism, which include the recombinogenic donor are then selected.

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