Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1994-11-29
1996-12-31
Kishore, Gollamudi S.
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
536127, 536124, C07H 100, C08B 3700
Patent
active
055895913
ABSTRACT:
This invention is composition comprising a highly purified, substantially endotoxin-free polysaccharide preparation suitable for use as a parenterally administered pharmaceutical, and the method for producing the composition. The process produces a substantially endotoxin-free polysaccharide composition using a size separation technique in which the low molecular weight impurities are removed first, with a subsequent separation of the endotoxin from the polysaccharide, again, by size separation. Suitable size separation techniques include gel fitration, or more preferably, ultrafiltration. In the preferred embodiment of the invention, the polysaccharide arabinogalactan is first ultrafiltered using a 10,000 dalton membrane; the low molecular weight impurities (<10,000 da) are discarded. The retentate is ultrafiltered using a 100,000 dalton membrane; the high molecular weight retentate, which contains the endotoxin, is discarded, leaving a substantially purified endotoxin free arabinogalactan composition in the filtrate. Other polysaccharides such as dextran, mannan, and gum arabic are also well suited for this purification technique. The process and products produced thereby are well suited for use in parenteral pharmacuetical compositions.
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Advanced Magnetics Inc.
Kishore Gollamudi S.
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