Endothelin DNA and use thereof

Details Endothelin DNA and use thereof Endothelin DNA and use thereof

1993-01-27

1994-03-15

Hill, Jr., Robert J.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

530324, 530326, 4352523, 4353201, 435 691, C07H 1512, C12P 2106, C12N 122

Patent

active

052945693

ABSTRACT:
Disclosed are (1) a DNA sequence containing a segment coding for endothelin-2 or human endothelin-3, (2) a precursor protein and a mature peptide of endothelin-2 or human endothelin-3, (3) a transformant carrying a DNA sequence containing a DNA segment coding for endothelin-2 or human endothelin-3 and (4) a method for preparing mature endothelin-2 or endothelin-3 which comprises culturing the transformant described in (3), producing and accumulating a protein in a culture medium, and collecting the same.
Cells transfected or transformed with the DNA allow large amounts of endothelin-2 or endothelin-3 to be produced, which causes the advantageous production of endothelin-2 or endothelin-3.
Endothelin-2 and endothelin-3 can be utilized as hypotension therapeutic agents or local vasoconstrictors to animals including humans.

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