Endothelial cell growth factor

Details Endothelial cell growth factor Endothelial cell growth factor

1989-11-06

1993-08-10

Lee, Lester L.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Hormones, e.g., prolactin, thymosin, growth factors, etc.

C07R 1300, C07K 1506

Patent

active

052350428

ABSTRACT:
Endothelial cell growth factor (ECG) from various sources possesses a strong and specific affinity for heparin. This strong affinity of ECG for heparin enables removal of undesired impurities from a mixture comprising ECG by: a) contacting immobilized heparin with the mixture to form a heparin-ECG complex; b) separating uncomplexed mixture from the complex; and c) contacting the complex with a salt solution of a salt concentration and pH effective to separate the ECG from the heparin. The resulting purified ECG (or fragment thereof) is useful in therapeutics and as an additive for cell culturing. The purified ECG is also useful to raise antibodies that are used in therapeutics and in ECG immunoassays.

REFERENCES:
patent: 4785079 (1988-11-01), Gospodarowicz et al.
patent: 4902782 (1990-02-01), Gospodarowicz et al.
patent: 4956455 (1990-09-01), Esch et al.
patent: 4994559 (1991-02-01), Moscatelli et al.
patent: 5026839 (1991-06-01), Moscatelli et al.
Marx, J., "Holding the Line Against Heart Disease", Science, 248: 1491-1493, Jun. 1990.
Shing et al. (1985) J. Cell Biochem 29:275-287.
Maciag et al. (1982) J. Biol. Chem.
Thomas et al. (1984), Proc. Nat'l. Acad. Sci., USA 81, 357-361.
Klagsburn (1980) J. Biolog. Chem. 225:10859-10864.
LaBrecque et al. (1982) Fed. Proc. p. 620 Abstract #2051.
Heparin-Sepharose CL-6.
Shing et al. (1983) Abstracts, 74th Annual Meeting of Am. Soc. of Biol. Chemists, No. 418, p. 1829.
Shing et al. (1983) Abstracts, 23rd Annual Meeting of the Am. Soc. for Cell Biol. No. 1488, p. 395a.
Clemmons et al. (1983) Proc. Nat'l Acad. Sci 80 1641-1645.
Sullivan and Klagsbrun (1985) J. Biolog. Chem. 260(4):2399-2403.
D'Amore and Klagsbrun (1985) J. Cell Biol. 99:1545-1549.
Lobb et al. J. Biol. Chem. 261(4):1924 (1986).
Maciag et al. (1984) Science 225:932-934.
Conn and Hatcher (1984) Biochem. and Biophys. Res. Comm. 124(1):262-268.
Lobb and Fett (1984) Biochem. 23:295.
Gospodarowicz et al. (1985) Biochem. and Biophys. Res. Comm. 128(2):554-562.
Bohen et al. (1984) Proc. Nat'l Acad. Sci. 81:5364-5368.
Bohlen et al. (1985) J. Cell Biochem (Supp) 9a:133.
Esch et al. (1985) Proc. Nat'l Acad. Sci. 82:6507-6511.
Gimenez-Gallego et al. (1985) Science 230 (4732:1385-1389.
Bohlen et al. FEBS 185 (1)177-181.
Burgess et al., Ann. Rev. Biochem. 575, 577 (1989).
Gospodarowicz, Nature 249:123 (1974).
Gospodarowicz, J. Biol. Chem. 250:2515 (1975).
Gospodarowicz, J. Biol. Chem. 253:3726 (1978).
Westall et al., PNAS USA 75:4675 (1978).
Gospodarowicz et al., J. Biol. Chem. 257:12266 (1982).
Shing et al., Science 223:1296 (1984) (Mar.).
Gospodarowicz et al., PNAS USA 81:6963 (1984) (Nov.).
Klagsbrun et al., PNAS USA 82:805-809 (1985).
Thomas FASEB J. 1:434 (1987).
Lobb, Eur. J. Clin. Invest. 18:321 (1988).
Gospodarowicz, Crit. Rev. Oncog. 1:1 (1989).
Lehninger, Biochemistry 2d. Ed. 162 (1977).
Lehninger, Principles of Biochemistry 144-145 (1982).
Connolly et al., J. Clin. Invest. 84:1470-1478 (1989).
Ferrara et al., Biochem. Biophys. Res. Com. 161:851-858 (1989).
Tischer et al., Biochem. Biophys. Res. Com. 165:1198-1206 (1989).

Affiliated with

Also associated with

Rating

Endothelial cell growth factor does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Endothelial cell growth factor, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Endothelial cell growth factor will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1726351