Endoproteinase-Lys-C and process for its preparation thereof

Details Endoproteinase-Lys-C and process for its preparation thereof Endoproteinase-Lys-C and process for its preparation thereof

1981-08-28

1983-11-08

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435822, C12N 950, C12R 101

Patent

active

044143320

ABSTRACT:
The present invention provides endoproteinase-Lys-C from bacteria, consisting of a chain of molecular weight 35000 to 38000 Dalton, having a pH optimum at pH 7.7 and being inhibited by aprotinin but not being inhibited by alpha.sub.2 -macroglobulin, .alpha..sub.1 -antitrypsin and ethylenediamine-tetraacetic acid.
The present invention also provides a process for obtaining this enzyme, wherein a culture broth of an appropriate bacterial strain which has been filtered or pre-purified by usual methods is chromatographed over carrier-fixed alpha.sub.2 -macroglobulin-metal complex and the enzyme is obtained from the filtrate.

REFERENCES:
Stone et al., Journal of Biological Chemistry, vol. 254, No. 21, pp. 10857-10861, Nov. 10, 1979.

Affiliated with

Also associated with

Rating

Endoproteinase-Lys-C and process for its preparation thereof does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Endoproteinase-Lys-C and process for its preparation thereof, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Endoproteinase-Lys-C and process for its preparation thereof will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2008693