Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1992-11-17
1994-02-22
Naff, David M.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
4352547, 435929, 25217412, 252DIG12, C12N 958, C12N 114, C12N 100, C11D 3386
Patent
active
052886271
ABSTRACT:
An endoprotease preparation, comprising an isolated endoprotease is disclosed. The endoprotease is a serine protease, shows immunochemical idenity to a protease derived from Fusarium oxysporum DSM 2672, hydrolyzes the oxidized beta-chain of bovine insulin at the peptide bonds Arg (22)-Gly (23) and Lys (29)-Ala(30), has optimum activity towards casein in the pH range of 8.5-11.0 with nearly constant activity in the pH range, has optimum activity at a temperature of about 45.degree. C., and has an isolectric point of about 9.0-10.0. The preferred microorganism that the enzyme is isolated from is Fusarium oxysporum DSM 2672.
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Aaslyng Dorrit A.
Jensen Georg W.
Nielsen Ruby I.
Schneider Palle
Lambiris Elias J.
Meller Michael V.
Naff David M.
Novo Nordisk A S
Zelson Steve T.
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