Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification
Reexamination Certificate
2011-03-01
2011-03-01
Nashed, Nashaat T (Department: 1656)
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
C435S325000, C435S410000, C435S252300, C435S254110, C435S320100, C530S324000, C536S023100
Reexamination Certificate
active
07897394
ABSTRACT:
The invention relates to cellular localization signals. In particular, the invention relates to endoplasmic reticulum localization signals in monomeric or multimeric form. The localization signals are utilized as research tools or are linked to therapeutics. Disclosed are methods of making and using polypeptides and modified polypeptides as signals to localize therapeutics, experimental compounds, peptides, proteins and/or other macromolecules to the endoplasmic reticulum of eukaryotic cells. The polypeptides of the invention optionally include linkage to reporters, epitopes and/or other experimental or therapeutic molecules. The invention also encompasses polynucleotides encoding the localization signals and vectors comprising these polynucleotides.
REFERENCES:
patent: 7071295 (2006-07-01), Reed
patent: 2004/0185556 (2004-09-01), Reed
patent: 2008/0050808 (2008-02-01), Reed et al.
patent: WO 2005/040336 (2005-05-01), None
patent: WO 2005/116231 (2005-12-01), None
Andersson, et al. 1999 Protein targeting to endoplasmic reticulum by dilysine signals involves direct retention in addition to retrieval J Biol Chem 274:15080-4.
Cocquerel, et al. 1999 The transmembrane domain of hepatitis C virus glycoprotein E1 is a signal for static retention in the endoplasmic reticulum J Virol 73:2641-9.
Fons, et al. 2003 Substrate-specific function of the translocon-associated protein complex during translocation across the ER membrane J Cell Biol 160:529-39.
Gabathuler, et al. 1990 The endoplasmic reticulum retention signal of the E3/19K protein of adenovirus type 2 consists of three separate amino acid segments at the carboxy terminus J Cell Biol 111 :1803-10.
Honsho, et al. 1998 Retention of cytochrome b5 in the endoplasmic reticulum is transmembrane and luminal domain-dependent J Biol Chem 273:20860-6.
Ma, et al. 2002 A 15-residue bifunctional element in D-AKAP1 is required for both endoplasmic reticulum and mitochondrial targeting J Biol Chem 277:27328-36.
Mitoma, et al. 1992 The carboxy-terminal 10 amino acid residues of cytochrome b5 are necessary for its targeting to the endoplasmic reticulum Embo J 11:4197-203.
Mziaut, et al. 1999 Targeting proteins to the lumen of endoplasmic reticulum using N-terminal domains of 11lbeta-hydroxysteroid dehydrogenase and the 50-kDa esterase J Biol Chem 274:14122-9.
Parker, et al. 2004 Targeting of inositol 1,4,5-trisphosphate receptors to the endoplasmic reticulum by multiple signals within their transmembranc domains J Biol Chem 279:23797-805.
Pottekat, et al. 2004 Subcellular localization and targeting of N-acetylglucosaminyl phosphatidylinositol de-N-acetylase, the second enzyme in the glycosylphosphatidylinositol biosynthetic pathway J Biol Chem 279:15743-51.
Ren, et al. 2003 Cell surface expression of G1uR5 kainate receptors is regulated by an endoplasmic reticulum retention signal J Biol Chem 278:52700-9.
Szczesna-Skorupa, et al. 2001 The juxtamembrane sequence of cytochrome P-450 2C1 contains an endoplasmic reticulum retention signal J Biol Chem 276:45009-14.
Vainauskas, et al. 2005 Endoplasmic reticulum localization of Gaa1 and PIG-T, subunits of the glycosylphosphatidylinositol transamidase complex J Biol Chem 280:16402-9.
Watanabe, et al. 1996 PIG-A and PIG-H, which participate in glycosylphosphatidylinositol anchor biosynthesis, form a protein complex in the endoplasmic reticulum J Biol Chem 271:26868-75.
Zarei, et al. 2004 An endoplasmic reticulum trafficking signal prevents surface expression of a voltage- and Ca2+-activated K+ channel splice variant Proc Natl Acad Sci U S A 101:10072-7.
Zarei, et al. 2001 A novel MaxiK splice variant exhibits dominant-negative properties for surface expression J Biol Chem 276:16232-9.
Intrexon Corporation
Nashed Nashaat T
Sterne Kessler Goldstein & Fox P.L.L.C.
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