Endoglycanase isolated from bacillus ATCC 55294

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

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43525231, 435832, C12N 924, C12N 120, C12N 100

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active

053427734

ABSTRACT:
An isolated endoglycanase having activity against polysaccharides having the subunit backbone structure: ##STR1## wherein Glc is glucose, GlcA is glucuronic acid, Rha is rhamnose, Man is mannose, X may be Rha or Man, and wherein the reducing end of the polymer is toward the X residue of the backbone is disclosed. A method for producing the endoglycanase by culturing Bacillus ATCC 55294, the method for selecting the Bacillus ATCC 55294 and method for using the endoglycanase are also disclosed. The endoglycanase has a molecular weight of 110,000.+-.10,000 daltons and a pH range from 4.5 to 9.0.

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