Endoglucanases

Details Endoglucanases Endoglucanases

2007-06-05

2007-06-05

Slobodyansky, Elizabeth (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

C435S263000, C435S264000, C435S267000, C510S320000, C510S321000, C536S023200

Reexamination Certificate

active

10965499

ABSTRACT:
The present invention relates to enzyme preparations consisting essentially of an enzyme which has cellulytic activity and comprises a first amino acid sequence having the following sequence(SEQ ID NO: 79)ThrArgXaaXaaAspCysCysXaaXaaXaaCysXaaTrpXaa1234567891011121314and a second amino acid sequence having the following sequenceTrpCysCysXaaCys(SEQ ID NO: 80)12345wherein, at position 3 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 4 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 8 of the first sequence, the amino acid is Arg, Lys or His; at positions 9, 10, 12 and 14, respectively, of the first sequence, and at position 4 of the second sequence, the amino acid is any of the 20 naturally occurring amino acid residues with the provisos that, in the first amino acid sequence, (i) when the amino residue at position 12 is Ser, then the amino acid residue at position 14 is not Ser, and (ii) when the amino residue at position 12 is Gly, then the amino acid residue at position 14 is not Ala, performs very well in industrial applications such as laundry compositions, for biopolishing of newly manufactured textiles, for providing an abraded look of cellulosic fabric or garment, and for treatment of paper pulp. Further, the invention relates to DNA constructs encoding such enzymes, a method for providing a gene encoding for such enzymes, a method of producing the enzymes, enzyme preparations containing such enzymes, and the use of these enzymes for a number of industrial applications.

REFERENCES:
patent: 4081328 (1978-03-01), Skinner et al.
patent: 4966850 (1990-10-01), Yu et al.
patent: 5314637 (1994-05-01), Saito et al.
patent: 307564 (1989-03-01), None
patent: 495257 (1992-07-01), None
patent: 508358 (1992-10-01), None
patent: 1368599 (1970-09-01), None
patent: WO 90/02790 (1990-03-01), None
patent: WO 91/10732 (1991-07-01), None
patent: WO 91/17243 (1991-11-01), None
patent: WO 91/17244 (1991-11-01), None
patent: WO 93/20193 (1993-10-01), None
patent: WO 94/07998 (1994-04-01), None
patent: WO 94/21801 (1994-09-01), None
patent: WO 94/26880 (1994-11-01), None
patent: WO95/02043 (1995-01-01), None
patent: WO 95/24471 (1995-09-01), None
patent: WO 95/26398 (1995-10-01), None
Gerrit Beldman et al., European Journal of Biochemistry, vol. 146, pp. 301-308 (1985).
Henrissat et al., Cellulose, vol. 1, pp. 169-196 (1996).
Beguin, Annu. Rev. Microbiol., vol. 44, pp. 219-248 (1990).
Henrissat et al., Biochemical Journal, vol. 280, pp. 309-316 (1991).
Saloheimo et al., Gene, vol. 63, pp. 11-21 (1988).
Penttilä et al., Gene, vol. 45, 253-263 (1986).
Ali et al., Fems Microbiology Letters, vol. 25, pp. 15-22 (1995).
Zhou et al., Biochemical Journal, vol. 297, pp. 359-364 (1994).
Beguin et al., Fems Microbiology Reviews, vol. 13, pp. 25-58 (1994).
Sheppard et al., Gene, vol. 150, pp. 163-167 (1994).
Wang et al., Gene, vol. 58, pp. 125-128 (1995).
Xue et al., Journal of General Microbiology, vol. 138, pp. 1413-1420 (1992).
Wang et al., Applied and Environmental Microbiology, vol. 61, Part 5, pp. 2004-2006 (1995).
Xue et al., Journal Of General Microbiology, vol. 138, pp. 2397-2403 (1992).
Dalbøge and Heidt-Hansen, Molecular and General Genetics, vol. 243, pp. 253-260 (1994).
Saloheimo et al., Molecular Microbiology, vol. 32, Part 2, pp. 219-228 (1994).
Ooi et al., Nucleic Acids Research, vol. 18, Part 19, p. 5884 (1990).
Van Ardsell et al., Bio/Technology Research, vol. 5, pp. 60-64 (1987).
Enari et al., Microbial Celluloses, pp. 183-223.
Gonzalez et al., Applied Microbiology Biotechnology, vol. 38, pp. 370-375 (1992).
Yamane et al., Methods in Enzymology, vol. 160, pp. 200-391 (1988).
Ooi et al., Current Genetics, vol. 18, pp. 217-222 (1990).
Jones et al., Proc Natl Acad Sci U S A, vol. 88, pp. 4171-4175 (1991).
Damjanovski Sashko et al., Biochemical Journal, vol. 281, pp. 513-517 (1992).
Hayashida et al., Methods of Enzymology, vol. 160, pp. 323-332.
Alfonso et al., FEMS Microbiology Letters, vol. 99, pp. 169-174 (1992).

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