Endoglucanases

Details Endoglucanases Endoglucanases

2005-02-15

2005-02-15

Slobodyansky, Elizabeth (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

C435S263000, C435S264000, C510S320000, C510S321000, C536S023200

Reexamination Certificate

active

06855531

ABSTRACT:
The present invention relates to enzyme preparations consisting essentially of an enzyme which has cellulytic activity and comprises a first amino acid sequence having the following sequence(SEQ ID NO:79)Thr Arg Xaa Xaa Asp Cys Cys Xaa Xaa1   2   3   4   5   6   7   8   9Xaa Cys Xaa Trp Xaa10  11  12  13  14and a second amino acid sequence having the following sequenceTrp Cys Cys Xaa Cys (SEQ ID NO:80)1   2   3   4   5wherein, at position 3 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 4 of the first sequence, the amino acid is Trp, Tyr or Phe; at position 8 of the first sequence, the amino acid is Arg, Lys or His; at positions 9, 10, 12 and 14, respectively, of the first sequence, and at position 4 of the second sequence, the amino acid is any of the 20 naturally occurring amino acid residues with the provisos that, in the first amino acid sequence, (i) when the amino residue at position 12 is Ser, then the amino acid residue at position 14 is not Ser, and (ii) when the amino residue at position 12 is Gly, then the amino acid residue at position 14 is not Ala, performs very good in industrial applications such as laundry compositions, for biopolishing of newly manufactured textiles, for providing an abraded look of cellulosic fabric or garment, and for treatment of paper pulp. Further, the invention relates to DNA constructs encoding such enzymes, a method for providing a gene encoding for such enzymes, a method of producing the enzymes, enzyme preparations containing such enzymes, and the use of these enzymes for a number of industrial applications.

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