Endogenous ketogulonigenium plasmid

Details Endogenous ketogulonigenium plasmid Endogenous ketogulonigenium plasmid

2001-04-05

2003-01-07

Yucel, Remy (Department: 1636)

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

C435S320100, C536S023100, C536S024100

Reexamination Certificate

active

06503748

ABSTRACT:

BACKGROUND OF THE INVENTION
1. Field of the Invention
The present invention relates, in general, to an isolated or purified Ketogulonigenium plasmid endogenous to microorganism strain NRRL B-30035 (ADM 291-19).
2. Background Information
The exploitation of microorganisms to synthesize vitamin C or its chemical pathway intermediates has both economic and ecological advantages. One key intermediate in vitamin C synthesis is 2-keto-L-gulonic acid (2-KLG), which is easily converted chemically to L-ascorbic acid (vitamin C) by esterification followed by lactonization (Delic, V. et al., “Microbial reactions for the synthesis of vitamin C (L-ascorbic acid,” in
Biotechnology of Vitamins, Pigments and Growth Factors
, Vandamme, E. J., ed., Elsevier Applied Science (London & New York) pp. 299-336 (1989)). Members of a number of bacterial genera have been identified that produce 2-KLG from the oxidation of L-sorbose. Such 2-KLG producing genera include the acidogenic, alpha-proteobacteria Gluconobacter and Acetobacter, the gamma-proteobacteria Pseudomonas, Escherichia, Klebsiella, Serratia and Xanthmonas, the Gram positive Bacillus, Micrococcus, and the unofficial genus Pseudogluconobacter (Imai, K. etal., U.S. Pat. No. 4,933,289 (1990), Sugisawa, H. et al., “Microbial production of 2-keto-L-gulonic acid from L-sorbose and D-sorbitol by
Gluconobacter melanogenus,” Agric. Biol. Chem.
54:1201-1209 (1990), Yin, G. et al., U.S. Pat. No. 4,935,359 (1990) and Nogami, I. et al., U.S. Pat. No. 5,474,924 (1995)).
To aid in increasing the yield of bacterial products, attempts have been made to exploit endogenous plasmids within microorganism strains. (Beppu, T. et al., U.S. Pat. No. 5,580,782 (1996), Fujiwara, A. et al., U.S. Pat. No. 5,399,496 (1995)).
SUMMARY OF THE INVENTION
One aspect of the invention provides an isolated or purified nucleic acid molecule comprising a polynucleotide having a nucleotide sequence at least 95% identical to a sequence selected from the group consisting of: a nucleotide sequence in SEQ ID NO: 1; a nucleotide sequence of an endogenous plasmid contained in NRRL Deposit No. B-30035; and a nucleotide sequence complementary to any of the above.
Further embodiments of the invention include isolated nucleic acid molecules that comprise a polynucleotide having a nucleotide sequence at least 90% identical, and more preferably at least 95%, 97%, 98% or 99% identical, to any of the above nucleotide sequences, or a polynucleotide which hybridizes under stringent hybridization conditions to a polynucleotide having a nucleotide sequence as in the above. The polynucleotide which hybridizes does not hybridize under stringent hybridization conditions to a polynucleotide having a nucleotide sequence consisting of only A residues or of only T residues.
Further advantages of the present invention will be clear from the description that follows.


REFERENCES:
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patent: 4935359 (1990-06-01), Yin et al.
patent: 5399496 (1995-03-01), Fujiwara et al.
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Urbance, J.W. et al., “Taxonomic characterization ofKetogulonigenium vulgaregen. nov., sp. nov. andKetogulonigenium robustumsp. nov., which oxidize L-sorbose to 2-keto-L-gulonic acid,”Int. J. Systematic Evol. Microbiol.51 :1059-1070, Society for General Microbiology (May 2001).
Vandamme, E.J., “Production of Vitamins, Coenzymes and Related Biochemicals by Biotechnological Processes,”J. Chem. Tech. Biotechnol.53:313-327, Elsevier Applied Science (1992).
Claim as pending Apr. 9, 2002 in U.S. patent application No. 09/684,970, Stoddard et al., as filed Oct. 10, 2000; a divisional of U.S. application No. 09/290,234, now U.S. patent No. 6,319,699 (document AI1).
Claims as pending Apr. 9, 2002 in U.S. patent application No. 09/722320, Stoddard et al., filed Nov. 28, 2000: a divisional of U.S. patent application No. 09/393,655, now U.S. patent 6,316,231 (document AH1).
Claims as pending Apr. 9, 2002 in U.S. patent application No. 09/722,514, Stoddard et al., filed Nov. 28, 2000: a divisional of U.S. patent application No. 09/393,655, now U.S. patent 6,316,231 (document AH1).
Claims as pending Apr. 9, 2002 in U.S. patent application No. 09/722,427, Stoddard et al., filed Nov. 28, 2000: a divisional of U.S. patent application No. 09/393,655, now US patent 6,316,231 (document AH1).
U.S. patent application No. 09/826,206, D'Elia, J., filed Apr. 5, 2001.
U.S. patent application No. 09/826,191 (pending), D'Elia, J. et al., filed Apr. 5, 2001.
Delic, V., et al., “Microbial Reactions for the Synthesis of Vitamin C (L-Ascorbic Acid),” inBiotechnology of Vitamins, Pigments and Growth Factors, Vandamme, E.J., ed., Elsevier Applied Science (London & New York) pp. 299-336 (1989).
Sugisawa, H., et al., “Microbial Production of 2-Keto-L-Gulonic Acid from L-Sorbose and D-Sorbitol byGluconobacter melanogenus,” Agric Biol. Chem. 54:1201-1209, Japan Society for Bioscience, Biotechnology, and Agrochemistry (1990).

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