Endochitinase gene induced by osmotic stress and abscisic acid i

Details Endochitinase gene induced by osmotic stress and abscisic acid i Endochitinase gene induced by osmotic stress and abscisic acid i

1993-12-07

1997-08-12

Patterson, Jr., Charles L.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

4352523, 4353201, 4351723, 536 232, C12N 944, C12N 1556

Patent

active

056564740

ABSTRACT:
Two osmotic stress- and ABA-responsive members of the endochitinase (EC 3.2.1.14) gene family has been isolated and identified from leaves of drought-stressed Lycopersicon chilense plants. The 966-base-pair insert of pcht28 encodes an acidic chitinase precursor with an amino-terminal signal peptide. The mature protein is predicted to have 229 amino acid residues with a relative molecular weight of 24,943 and pI value of 6.2. The same number in amino acids, molecular and PI value are predicted for the protein encoded by pchtI, despite of a slight variation in the DNA and amino acid sequences. Sequence analysis revealed that pcht28 and pchtI have a high degree of homology with class II chitinases (EC 3.2.1.14) from tobacco. Northern blot analysis indicated that these genes have evolved a completely different pattern of expression from others reported thus far. They are highly induced by both osmotic stress and the plant hormone abscisic acid. Southern blot analysis of genomic DNA suggested that the pcht28- and pchtI-related chitinase is encoded by a small multigene family in this species. Knowing the role of plant chitinase in plant defense against fungal pathogens, it is assumed that, besides their general defensive function, the pcht28- and pchtI-encoded chitinases may play a particular role in protecting plants from pathogen attack during water stress.

REFERENCES:
GenBank entry M97210, (Jun. 7, 1993).
M.H.A.J. Joosten et al., "Identification of Several Pathogenesis-Related Proteins in Tomato Leaves Inoculated with Cladosporium-fulvum as 1,3-.beta.-Glucanases and Chitinases", 89(3) 945-951, (1989).
N. Danhash et al., "Molecular Characterization of Four Chitinase cDNAs Obtained From Cladosporium-fulvum-Infected Tomato", 22(6) 1017-1029 (Sep. 1993).
Baker, J. et al. (1988). Plant Molecular Biology. 11: 277-291.
Boller, T. (1988). Oxford Surveys of Plant Molecular & Cell Biology 5: 145-174.
Bray, E.A. (1988). Plant Physiol. 88: 1210-1214.
Chen, R.D. and Tabaeizadeh, Z. (1992a). Genome 35: 385-391.
Chen, R.-D. and Tabaeizadeh, Z. (1992b). Biochem. Cell Biol. 70: 199-206.
Chen, R.-D. et al. (1993). Plant Physiol. 103: 301.
Clarke, J.M. and Durley, R.C. (1981). "The Response of Plants to Drought Stress" In Water Stress on Plants, (Simpson, G.M., ed.) Praeger, New York, pp. 89-139.
Devereux, J. et al. (1984). Nucleic Acids Research. 12(1): 387-391.
Fisher, R.A. and Turner, N.C. (1978). Ann. Rev. Plant Physiol. 29:277-317.
Gaynor, J.J. (1988). Nucleic Acids Research: 16(11): 5210.
Gomez, J. et al. (1988). Nature. 334: 262-264.
Greer, A.F. and Tabaeizadeh, Z. (1991). Can. J. Bot. 69: 2257-2260.
Hanson, A.D. and Hitz, W.D. (1982). Ann. Rev. Plant Physiol. 33: 163-203.
Leah, R. et al. (1991). Journal of Biological Chemistry. 266(25): 1564-1573.
Maniatis, T. et al. (1982). Molecular Cloning: A laboratory Manual (Cold Spring Harbor Laboratory, Cold Spring Harbor, NY).
Mauch, F. et al. (1988). Plant Physiol. 88: 936-942.
Memelink, J. et al. (1990). Plant Molecular Biology. 14: 119-126.
Metraux, J.P. (1989). Proc. Natl. Acad. Sci. USA. 86: 896-900.
Mundy, J. and Chua, N.-H. (1988). EMBO Journal 7(8): 2279-2286.
Neale, A. D. et al. (1990). The Plant Cell. 2: 673-684.
Parent, J.-G. et al. (1985). Can. J. Bot. 63: 928-931.
Payne, G. et al. (1990). Proc. Natl. Acad. Sci. USA. 87: 98-102.
Sachs, M.M. and HO, T.-H. D. (1986), Ann. Rev. Plant Physiol. 37: 363-376.
Sanger, F. et al. (1977). Proc. Natl. Acad, Sci. USA. 74(12): 5463-5467.
Schlumbaum, A. et al. (1986). Nature. 324:365-367.
Skriver, K. and Mundy, J. (1990). The Plant Cell. 2: 503-512.
Shinshi, H. et al. (1990) Plant Molecular Biology. 14: 357-368.
Simpson, G.M. (1981). "The Value of Physiological Knowledge of Water Stress in Plants", In Water Stress on Plants, (Simpson, G.M., Ed), Praeger, NY, pp. 235-265.
Trudel, J. and Asselin, A. (1989). Analytical Biochemistry. 178: 362-366.
Ward, E. R. et al. (1991). The Plant Cell. 3: 1085-1094.
Wu, M.M.J., et al. (1983). Current Genetics. 7: 385-392.

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