Endo-1,4-.beta.-glucanase genes and their use in plants

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

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536 232, 536 236, 536 241, 4351723, 4352404, 4353201, 435209, C07H 1512, C12N 1500, C12N 500, C12N 942

Patent

active

053289994

ABSTRACT:
The present invention provides a method for reducing fruit softening and cell wall polysaccharide degradation by inhibiting endo-1,4-.beta.-glucanase activity using antisense DNA constructions.

REFERENCES:
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Sheehy, R. E., et al., "Reduction of polygalacturonase activity in tomato fruit by antisense RNA," Proc. Nat. Acad. Sci., 85:8805-8809 (1988).
Smith, C. J. S., et al., "Inheritance and effect on ripening of antisense polygalacturonase genes in transgenic tomatoes" Plant Molecular Biology, 14:369-379 (1990).
Hatfield, R., and Nevins, D. J., "Characterization of the Hydrolytic Activity of Avocado Cellulase", Plant Cell Physiol., 27(3):541-552 (1986).
Christoffersen, R. E., et al., "Cellulase gene expression in ripening avocado fruit: The accumulaton of cellulase mRNA and protein as demonstrated by cDNA hybridizaton and immunodetection", Plant Molecular Biol., 3:385-391 (1984).
Tucker, M. L., et al., "Bean Abscission Cellulase", Plant Physiol., 88:1257-1262 (1988).
Sobotka, F. E. and Watada, A. E., "Cellulase in High Pigment and Crimson Tomato Fruit", J. Amer. Soc. Hort. Sci. 96(6):705-707 (1971).
Bennett, A. L. and Christoffersen, R. E., "Synthesis and Processing of Cellulase from Ripening Avacado Fruit," Plant Physiol. 81:830-835 (1986).

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