Endo-1,4-.beta.-glucanase gene and its use in plants

Chemistry: molecular biology and microbiology – Vector – per se

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435 691, 435 698, 435 701, 4351723, 435209, 4352404, 935 48, 935 67, 536 27, C12N 1500, C12N 500, C12N 942, C12P 2104, C07H 1512

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051680645

ABSTRACT:
The present invention provides a method for reducing fruit softening and cell wall polysaccharide degradation by inhibiting endo-1,4-.beta.-glucanase activity using antisense DNA constructions.

REFERENCES:
patent: 4801540 (1989-01-01), Hiatt et al.
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Smith, C. J. S., et al., "Antisense RNA inhibition of polygalacturonase gene expression in transgenic tomatoes," Nature, 334:724-726 (1988).
Sheehy, R. E., et al., "Reduction of polygalacturonase activity in tomato fruit by antisense RNA," Proc. Nat. Acad. Sci., 85:8805-8809 (1988).
Smith, C. J. S., et al., "Inheritance and effect on ripening of antisense polygalacturonase genes in transgenic tomatoes," Plant Molecular Biology, 14:369-379, (1990).
Hatfield, R., and Nevins, D. J., "Characterization of the Hydrolytic Activity of Avocado Cellulase," Plant Cell Physiol., 27(3):541-552 (1986).
Christoffersen, R. E., et al., "Cellulase gene expression in ripening avocado fruit: The accumulation of cellulase mRNA and protein as demonstrated by cDNA hybridization and immunodetection," Plant Molecular Biol., 3:385-391 (1984).
Tucker, M. L., et al., "Bean Abscission Cellulase," Plant Physiol., 88:1257-1262 (1988).
Sobotka et al., 1971, J. Amer. Soc. Hort. Sci. 96(6):705-707.
Bennett et al., 1986, Plant Physio. 81:830-835.
Sobotka et al., 1974 Plant Physiology 53:759-763.

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