End-column conductivity detector for capillary zone electrophore

Chemistry: electrical and wave energy – Apparatus – Coating – forming or etching by sputtering

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2041801, G01N 2726, G01N 27447

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active

052981398

ABSTRACT:
An end-column conductivity detector is described. A sensing electrode is placed in the outlet end of a capillary that has a hole in its side wall through which electrolyte moves past the sensing electrode into a reservoir that contains the ground electrode. This structure is simple to construct, has almost no dead volume, and minimizes electrolyte contact with adhesives used to mount the sensing electrode. The end-column conductivity detector is operated in conjunction with a commercial capillary zone electrophoresis system that has a capillary cartridge and a UV absorption detector. This design thus permits sequential measurement of the absorption and conductivity characteristics of separated analyte zones. When a species can be detected by both conductivity and UV absorption, then the absorption coefficient can be determined from the relation between the conductivity signal and the concentration of the species.

REFERENCES:
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patent: 4908116 (1990-03-01), Zare et al.
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Huang et al., "On-Column Conductivity Detector for Capillary Zone Electrophoresis", Anal. Chem., 59 (1987), 2747-2749.
Huang et al., "Effect of Electrolyte and Sample Concentration on the Relationship between Sensitivity and Resolution in Capillary Zone Electrophoresis using Conductivity Detection", J. Chromatogr., 480 (1989), 285-288.
Huang et al., "Quantitative Analysis of Low Molecular Weight Carboxylic Acids by Capillary Zone Electrophoresis/Conductivity Detection", Anal. Chem., 61 (1989), 766-770.
Kuhr, "Capillary Electrophoresis", Anal. Chem., 62 (1990), 403R-414R.
Foret et al., "On-Line Fiber Optic UV Detection Cell and Conductivity Cell For Capillary Zone Electrophoresis", Electrophoresis, 1986, 7, 430-432.
Huang et al., "Use of an On-Column Frit in Capillary Zone Electrophoresis: Sample Collection," Anal. Chem., 1990, 62, 443-446.
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