End-attachment of oligonucleotides to polyacrylamide solid suppo

Details End-attachment of oligonucleotides to polyacrylamide solid suppo End-attachment of oligonucleotides to polyacrylamide solid suppo

1989-01-05

1993-08-17

Brown, Johnnie R.

Synthetic resins or natural rubbers -- part of the class 520 ser

Synthetic resins

Mixing of two or more solid polymers; mixing of solid...

525340, 536 231, 536 254, 436501, C08F12056, C07H 2102, C07H 2104

Patent

active

052370160

ABSTRACT:
This invention concerns methods and means for covalent attachment of oligonucleotides to solid supports substantially at their 5'-ends. According to the invention thiol-oligonucleotides are attached to bromoacetyl-derivatized polyacrylamide supports, or conversely, bromoacetyl-oligonucleotides are immobilized on thiol-polyacrylamide supports.
In a further aspect, this invention relates to bromoacetyl-oligonucleotides that may be immobilized on thiol-polyacrylamide solid supports, thiol-oligonucleotides immobilized on bromoacetyl-derivatized polyacrylamide supports as well as to methods for capture of nucleic acids by oligonucleotides attached to polyacrylamide solid supports, either by direct capture or in sandwich hybridization formats.

REFERENCES:
Chu et al. (1983) "Derivatization of unprotected polynucleotides", Nucleic Acids Res. 11: 6513-6529.
Wilcheck et al., Biochemistry 26, 2155 (1987).
Wilcheck et al., PNAS 72, 1055 (1975).
Kremsky et al., Nucleic Acids Research 15, 2891 (1987).
Inman, Meth. Enzymol. 34, 30 (1969).
Bernatowitz et al., Anal. Biochem. 155, 95 (1986).
Ghosh et al., Nucl. Acids Res. 15, 5353 (1987).
Li et al., Nucl. Acids Res. 15, 5275 (1987).
Chu et al., Nucl. Acids Res. 14, 5591 (1986).
Connolly, Nucl. Acids Res. 13, 4485 (1985).
Keller et al., Helvetica Chimica Acta 58, 531 (1975).
Kwoh et al., Proc. Natl. Acad. Sci. USA 86 (1989).
Goodchild, Bioconjugate Chemistry 1(3): 165-186, 1990.
Chu et al., Nucl. Acids Res. 11(18): 6513-6529, 1983.

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