End-attachment of oligonucleotides to polyacrylamide solid suppo

Details End-attachment of oligonucleotides to polyacrylamide solid suppo End-attachment of oligonucleotides to polyacrylamide solid suppo

1995-03-31

1997-09-02

Jones, W. Gary

Synthetic resins or natural rubbers -- part of the class 520 ser

Synthetic resins

Mixing of two or more solid polymers; mixing of solid...

435 6, 435 5, 435 912, 435 911, C08F 2056, C12Q 168, C12Q 170, C12P 1934

Patent

active

056632428

ABSTRACT:
Methods for covalent attachment of oligonucleotides to solid supports such that substantially all of the oligonucleotides are attached via their 5'-ends are provided. The solid supports with attached oligonucleotides are produced. Thiol-oligonucleotides are attached to bromoacetyl-derivatized polyacrylamide supports, or conversely, bromoacetyl-oligonucleotides are immobilized on thiol-polyacrylamide supports. In a further aspect, bromoacetyl-derivatized oligonucleotides, and polyacrylamide supports with linked oligonucleotides produced by coupling bromoacetyl-derivatized oligonucleotides with thiol-derivatized polyacrylamide solid supports or by coupling thiol-derivatized oligonucleotides with bromoacetyl-derivatized polyacrylamide supports as well as methods for capture of nucleic acids by oligonucleotides attached to polyacrylamide solid supports, either by direct capture or in sandwich hybridization formats are provided.

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