Encoding antigens of M. Leprae

Details Encoding antigens of M. Leprae Encoding antigens of M. Leprae

1986-07-31

1990-03-06

Griffin, Ronald W.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 28, 536 29, C07H 19073

Patent

active

049067427

ABSTRACT:
Genes encoding five immunodeterminant protein antigens of the leprosy parasite Mycobacterium leprae have been isolated. The gene encoding the M. leprae 65kD antigen was sequenced and a lambda gt11 gene sublibrary was constructed with fragments of the gene. Recombinant DNA clones producing specific antigenic determinants were isolated using monoclonal antibodies and the sequences of their insert DNAs were determined with a rapid primer extension method. Amino acid sequences for six different epitopes of the M. leprae protein were elucidated. A peptide containing sequences for one of these epitopes, which is unique to M. leprae, was synthesized and shown to bind the appropriate monoclonal antibody; The approach described here can be used to elucidate rapidly protein epitopes that are recognized by antibodies or T cells. In addition, the well-characterized M. leprae antigens can be used in prevention, diagnosis and treatment of leprosy.

REFERENCES:
Young et al, Nature, 316, pp. 450-452, 1985 (Aug. 1).
Jacobs et al., Proc. Nat. Acad. Sci. U.S.A., vol. 83, pp. 1926-1930 (1986)-(Mar.).
Buchanan et al., Biological Abstracts/RRM, no. 30116549, (1986), vol. 86, no. O, p. 122.

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