Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;... – Enzyme or microbial cell is immobilized on or in an...
Patent
1995-11-08
1997-12-02
Naff, David M.
Chemistry: molecular biology and microbiology
Carrier-bound or immobilized enzyme or microbial cell;...
Enzyme or microbial cell is immobilized on or in an...
435182, 435382, C12N 1114, C12N 500
Patent
active
056935137
ABSTRACT:
Living tissue cells such as animal or plant tissue cell are encapsulated in an inorganic gel by mixing an organosilicon precursor with an aqueous acidic solution to form a gel forming solution and hydrolyze the organosilicon precursor, cooling the gel forming solution, forming a mixture of living tissue cells and Hank's balanced salt solution, adding a base solution to the gel forming solution to form a mixture, immediately thereafter adding the mixture containing living tissue cells to the mixture containing the gel forming solution, and pouring the resultant mixture into a container where an inorganic gel forms encapsulating the cells. The organosilicon precursor may be tetraethoxysilane, tetrabutoxysilane, tetramethoxysilane or tetrapropoxysilane.
REFERENCES:
patent: 4148689 (1979-04-01), Hino et al.
patent: 4391909 (1983-07-01), Lim
patent: 4797213 (1989-01-01), Parisius
patent: 5200334 (1993-04-01), Dunn et al.
Johansen W. Edward
Naff David M.
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