Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Fungi
Patent
1994-06-13
1996-03-05
Lilling, Herbert J.
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Fungi
4352521, 4352552, 25218628, 25218638, 25218641, 4284022, 264 41, B01J 1302, C11D 3395, C12N 116, C12N 118
Patent
active
054967287
DESCRIPTION:
BRIEF SUMMARY
FIELD OF THE INVENTION
The present invention relates to a method of reducing the odour of micro-organism cells. The invention also relates to the use of the resultant deodorised cells in a process for encapsulating a material, in which micro-organism cells are contacted with the said material, which material is in liquid form, whereby the said material is absorbed through the micro-organism cell wall and retained within the micro-organism cells. The invention also relates to liquids that have been encapsulated in that manner.
BACKGROUND TO THE INVENTION
The encapsulation of materials within micro-organism cells is well known. In EP-B-0,085,805 (Dunlop Limited), a method of encapsulation is described in which the micro-organism is contacted with an organic lipid-extending substance that is a solvent, or a micro-dispersing medium, for the material to be encapsulated, and simultaneously and/or subsequently the micro-organism is contacted with the material to be encapsulated, said material being employed as a solution or micro-dispersion in the organic lipid-extending substance, or in a further organic lipid-extending substance or in a liquid that is miscible with the first-mentioned lipid-extending substance, whereby both the organic lipid-extending substance and the material to be encapsulated are taken into and retained passively within the micro-organism. Suitable micro-organisms include yeasts and suitable lipid-extending substances include aliphatic alcohols, esters, aromatic hydrocarbons and hydrogenated aromatic hydrocarbons. An example of a material that can be encapsulated is a leuco dye suitable for use in "carbonless" copy paper. A stated advantage of the method described in that European patent is that, in contrast to certain earlier proposals (cf. U.S. Pat. No. 4,001,480 and FR-A-2,179,528), use may be made of micro-organisms having a natural lipid content of less than 40 percent by weight, without the need to employ a plasmolyser.
EP-A-0,242,135 (AD2 Limited) discloses a method of producing an encapsulated material by contacting the material in liquid form with a grown intact micro-organism having a microbial lipid content of less than 40 percent by weight. The encapsulatable material must be capable of diffusing into the microbial cell without causing total lysation thereof and the treatment of the micro-organism is carried out in the absence of an organic lipid-extending substance as solvent or microdispersant for the encapsulatable material and in the absence of a plasmolyser. The material is absorbed by the micro-organism--typically a yeast--by diffusion across the microbial cell wall and is passively retained within the micro-organism. A wide variety of encapsulatable materials are mentioned, including essential oils used as flavours or fragrances, leuco dyes, vitamins, detergents such as lauryl ether sulfate, food colourants, and pesticides and the like.
EP-A-0,414,282 (Quest International) discloses bleach compositions, including laundry detergents, laundry bleaches and dishwashing or scouring products, that contain a perfume whereas EP-A-0,414,283 (Quest International) discloses fabric-softening compositions that contain a perfume. In both cases, the perfume is encapsulated in micro-organism cells according to the conventional methods, as described in U.S. Pat. No. 4,001,480 or EP-A-0,242,135.
A problem that arises when using micro-organism cells for encapsulation purposes is that they may have a disagreeable odour and possibly also an unpleasing colour and may therefore diminish the acceptability of the encapsulated products, or compositions containing them, to consumers.
SUMMARY OF THE INVENTION
It has now been found that micro-organism cells may be at least partially deodorised by treatment with a peroxygen bleach whilst leaving the cells at least largely intact and hence suitable for encapsulation purposes.
The present invention, in one of its aspects, accordingly provides a method of reducing the odour of micro-organism cells, characterised in that the said cells are treated
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Hardy Frederick E.
Scialla Stefano
Willey Alan D.
Lilling Herbert J.
Rasser J. C.
Reed T. D.
The Procter & Gamble & Company
Zerby K. W.
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