Encapsulation of bioactive complexes in liposomes

Details Encapsulation of bioactive complexes in liposomes Encapsulation of bioactive complexes in liposomes

2000-03-01

2009-02-17

Kishore, Gollamudi (Department: 1612)

Drug, bio-affecting and body treating compositions

Preparations characterized by special physical form

Liposomes

C264S004100, C264S004300, C264S004600

Reexamination Certificate

active

07491409

ABSTRACT:
This invention provides a method to prepare liposome-encapsulated bioactive agents such as nucleic acids, comprising complexation of the bioactive agents in reverse micelles prior to forming liposomes, as well as methods of using the liposomes so formed and formulations to deliver nucleic acids to cells.

REFERENCES:
patent: 5286634 (1994-02-01), Stadler et al.
patent: 5723147 (1998-03-01), Kim et al.
patent: 5759573 (1998-06-01), Kim
patent: 5795587 (1998-08-01), Gao et al.
patent: 6071533 (2000-06-01), Papahadjopoulos et al.
Kim, Cancer Research, vol. 53, Apr. 1993, pp. 1596-1598.
Szoka et al in Proc. Natl. Acad. Sci., USA, vol. 75, #9, pp. 4194-4198, Sep. 1978.
Fraley et al Biochemistyr, 1981, vol. 20, pp. 6978-6987.
Ezio et al., “Solubilization and Structural Properties of Nucleic Acids in Reverse Micelles,” Controlled Release of Drugs: Polymers and Aggregate Systems, VCH Publishers, Inc., New York (1989), pp. 255-276, XP-001146370.
Ibanez et al., “Spermidine-condensed DNA and cone-shaped lipids improve delivery and expression of exogenous DNA transfer by liposomes,” Biochemistry and Cell Biology, 74(5):633-643 (1996).
Luisi et al., “Solubilization of Enzymes and Nucleic Acids in Hydrocarbon Micellar Solutions,” Critical Reviews in Biochemistry, 20(4):409-474 (1986).
Tikchonenko et al., “Transfer of condensed viral DNA into Eukaryotic cells using proteoliposomes,” Gene, 63(2):321-330 (1988).

Affiliated with

Also associated with

Rating

Encapsulation of bioactive complexes in liposomes does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Encapsulation of bioactive complexes in liposomes, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Encapsulation of bioactive complexes in liposomes will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4072181