Encapsidated recombinant poliovirus nucleic acid and methods of

Details Encapsidated recombinant poliovirus nucleic acid and methods of Encapsidated recombinant poliovirus nucleic acid and methods of

1996-01-22

1997-03-25

Elliott, George C.

Chemistry: molecular biology and microbiology

Vector, per se

435 693, 4351721, C12P 2102, C12N 1543

Patent

active

056144130

ABSTRACT:
The present invention pertains to a method of encapsidating a recombinant poliovirus nucleic acid to obtain a yield of encapsidated viruses which substantially comprises encapsidated recombinant poliovirus nucleic acid. The method of encapsidating a recombinant poliovirus nucleic acid includes contacting a host cell with a recombinant poliovirus nucleic acid which lacks the nucleotide sequence encoding at least a portion of a protein necessary for encapsidation and an expression vector comprising a nucleic acid which encodes at least a portion of one protein necessary for encapsidation under conditions appropriate for introduction of the recombinant poliovirus nucleic acid and the expression vector into the host cell and obtaining a yield of encapsidated viruses which substantially comprises an encapsidated recombinant poliovirus nucleic acid. A foreign nucleotide sequence is generally substituted for the nucleotide sequence of the poliovirus nucleic acid encoding at least a portion of a protein necessary for encapsidation. The invention further pertains to encapsidated recombinant poliovirus nucleic acids produced by the method of this invention and compositions containing the encapsidated recombinant poliovirus nucleic acid containing a foreign nucleotide sequence for use in a method of stimulating an immune response in a subject to the protein encoded by the foreign nucleotide sequence.

REFERENCES:
J.L. Fox (1994) Bio /Technology 12:128.
F.D. Ledley (1991) Human Gene Therapy 2:77-83.
A. Knuth et al. (1991) Current Opinion in Immunology 3:659-664.
D. C. Porter et al. (1993) J. Virology 67(7).
Porter D.C. et al. (Mar. 13-18 1993) "Encapsidation of Chimeric HIV-1-Poliovirus Minireplicons" J. Cell Biochem Suppl. 17 (D): p. 26.
Ansardi, D.C. et al. (Mar.13-18 1993) "Molecular Analysis of Poliovirus Assembly Using Recombinant Vaccina Viruses to Complement A Poliovirus Genome With A Capsid Gene Deletion" J. Cell Biochem. Suppl. 17 (D); p. 22.
Bass, J.W. et al. (1979) "Oral Polio Vaccine: Effect of Booster Vaccination One to 14 Years After Primary Series" JAMA 239(21); 2252-2255.
Ogra, P.L. and Karzon, D.T. (1971) "Formation and Function of Poliovirus Antibody in Different Tissues" Prog. Med. Virol. 13:156-193.
Conry, R.M. et al. (1995) J. Immunol. (Abstract from the Nov. 1-4, 1995 10th Annual Scientific Meeting of the Society for Biological Therapy, Williamsburg, VA).
Ansardi, D.C. et al. (1994) "Characterization of Poliovirus Replicons Encoding Carcinoembyonic Antigen" Cancer Research 54:6359-6364.
Kantor, J. et al. (1992) "Antitumor Activity and Immune Responses Induced by a Recombinant Carcinoembryonic Antigen-Vaccina Virus Vaccine" J. Natl. Cancer Institute 84:1084-1091.
Haynes, B.F. (1993) "Scientific and Social Issues of Human Immunodeficiency Virus Vaccine Development" Science 260:1279-1286.
Ansardi, D.C. et al. (1992) "Myristylation of Poliovirus Capsid Precursor P1 is Required for Assembly of Subviral Particles" J. Virol. 66(7):4556-4563.
McGhee, J.R. and Mestecky, J. (1992) "The Mucosal Immune System in HIV Infection and Prospects for Mucosal Immunity to AIDS" AIDS Res. Rev. 2:289-312.
Percy, N. et al. (1992) "A Poliovirus Replicon Containing the Chloramphenicol Acetyltransferase Gene Can Be Used To Study the Replication and Encapsidation of Poliovirus RNA" J. Virol. 66(8):5040-5046.
Ansardi, D.C. et al. (1991) "Coinfection with Recombinant Vaccina Viruses Expressing Poliovirus P1 and P3 Proteins Results in Polyprotein Processing and Formation of Empty Capsid Structures" J. Virol. 65(4):2088-2092.
Choi, W.S. et al. (1990) "Expression of Human Immunodeficiency Virus Type 1 (HIV-1) gag, pol, and env Proteins from Chimeric HIV-1-Poliovirus Minireplicons" J. Virol. 65(6):2875-2883.
Jenkins, O. et al. (1990) "An Antigen Chimera of Poliovirus Induces Antibodies Against Human Papillomavirus Type 16" J. Virol. 64(3):1201-1206.
Evans, D.J. et al. (1989) "An Engineered Poliovirus Chimera Elicits Broadly Reactive HIV-1 Neutralizing Antibodies" Nature 339:385-388.

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