Embryogenesis of gymnosperm forest trees

Details Embryogenesis of gymnosperm forest trees Embryogenesis of gymnosperm forest trees

1979-05-07

1980-08-19

Bagwill, Robert E.

Plant husbandry

Process

A01G 100

Patent

active

042177308

ABSTRACT:
Young Douglas-fir cotyledon tissue is cultured to grow a soft callus on an agar medium containing mineral salts, organic nutrients and an auxin. No cytokinins are present except those that might be endogenous in the tissue itself. After one to two months the explants and callus are placed in a liquid suspension culture medium similar to the first in composition. Again, auxins are present and cytokinins are omitted. After about one month, bipolar embryoids have been generated in the suspension. These have chloroplasts and a vascular system with organized meristemic centers for ultimate development into stems and roots. The embryoids can be removed from the culture and placed on a growth medium for production of plantlets.

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patent: 4152869 (1979-05-01), Jones
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Embryoids in-, Winton et al., Proc. TAPPI 1977 For. Biol. Wood Chem. Conf., Atlanta.
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