Embryogenesis in vitro, induction of qualitative and quantitativ

Plant husbandry – Process

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A01G 3100, C12K 900

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active

042415365

ABSTRACT:
The process of embryogenesis in vitro whereby single embryonic cells are obtained and from which normal, healthy plants can be produced. The process is capable of making, in vitro, any metabolite producible by the whole plant, for example tropane alkaloids. This process of embryogenesis in vitro comprises the steps of inducing from any plant material, for example, from plant material of the families Solanaceae and Erythroxylaceae, the formation of a partially dedifferentiated tissue comprised of mitotic cells, such as callus and tumor cells, preparing a cell culture therefrom, rejuvenating the cells to return them to the embryonic state through controlled environmental conditions, for example, in the case of Solanaceae and Erythroxylaceae through temperature and light, differentiating the embryonic cells to restore the ageing process thereof whereby the tissue function, as part of their normal growth pattern, will give embryos and consequently metabolites. At this point qualitative and quantitative changes in the pattern of the metabolites can be induced.
The process of producing a non-cyto-toxic-metabolite, from plant material.
The process of obtaining mixed fermentation of cultured cells from higher plants whereby a differentiated cell producing the messenger undergoes embryogenesis.
The process of inducing symbiotic relationship between cultured cells of higher plants and microorganisms for producing physiologically active compounds as for example anti-neoplastic agents.
The process of inducing tumor in plant by stimulating the endogenic hormone of the cells with an exogenic supply.
The novel products obtained by the foregoing processes.

REFERENCES:
patent: 2747334 (1956-05-01), Routien et al.
patent: 3704546 (1972-12-01), Hardy et al.
Growth and Development--, Steward et al., Science, vol. 143, Jan. 1964, pp. 20-27.
Haploid Plants--, Nitsch et al., Science, vol. 163, Jan. 1969, pp. 85-87.
Factors Influencing Morphogenesis--, Thomas et al., Ann. of Botany 36, 1972, pp. 239-247.
Role of the Generative--, Raghavan, Science, vol. 191, Jan. 1976, pp. 388-389.
Acta Botanica Sinica 20, Series 3, 1978, pp. 204-209.
Morphogenetic Responses--, Krikorian et al., Science, vol. 200, Apr. 1978, pp. 67-68.
Production of Natural Substances--Misana, Tokyo, Res. Lab., Kyowa Hakko Hogyo Co. Ltd., Machida-shi, Tokyo, Japan.

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