Embryo preferred promoter and method of using same

Details Embryo preferred promoter and method of using same Embryo preferred promoter and method of using same

2007-02-27

2007-02-27

Mehta, Ashwin (Department: 1638)

Chemistry: molecular biology and microbiology

Process of mutation, cell fusion, or genetic modification

Introduction of a polynucleotide molecule into or...

C435S419000, C435S320100, C536S024100, C800S281000, C800S287000

Reexamination Certificate

active

11127004

ABSTRACT:
A regulatory region is shown, a nucleotide sequence of approximately 3kb which provides improved seed preferred, and particularly embryo preferred expression in plants. Methods of use are also shown in preferentially expressing a heterologous protein to the embryo tissue of a plant. The sequence is particularly useful in expression of heterologous proteins to the embryo of monocotyledonous plants, particularly cereals, and maize.

REFERENCES:
patent: WO2001/36596 (2001-05-01), None
Whitelaw et al. (NCBI, GenBank, Accession No. CG000752, Published Aug. 19, 2003).
GenBank accession No. X55388; GI: 22270, submitted Nov. 4, 1990.
Williams and Tsang, “A maize gene expressed during embryogenesis is abscisic acid-inducible and highly conserved” Plant Mol. Biol. 16(5), 919-923 (1991).
Genbank accession No. AY104117 (GI: 21207195) submitted 2002.
Genbank accession No. AF544157 (GI:23395379) submitted Sep. 3, 2002.
GenBank accession No. AF544158 (GI: 23395380) submitted Sep. 3, 2002.
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Genbank accession No. X12564 (GI: 22312) submitted 1988.
GenBank accession No. AC146950, birren et al. Jan. 17, 2004.
GenBank accession No. CG000752, Whitelaw et al. Aug. 19, 2003.
Liu et al. “Tissue-specific and ABA-regulated Maize GIb1 gene expression in transgenic tobacco” Plant Cell Reports 1996, vol. 16: 158-162.

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