Elution method and device

Chemistry: electrical and wave energy – Processes and products – Electrostatic field or electrical discharge

Patent

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Details

2041801, 2041828, 204299R, 204301, C25B 700, C07K 314

Patent

active

049649612

ABSTRACT:
The present invention is directed to an apparatus for electro elution of components separated by preparative electrophoresis on a gel comprising a tapered tube divided by a porous disc into an upper section which is open at the top of the tapered tube and a lower section which is closable by a removable cap. The apparatus also contains a dialysis membrane of substantially the same diameter as the removable cap and affixed to the removable cap such that the dialysis membrane is sealed against the end of the tapered tube when the removable cap encloses the lower section of the tapered tube. Following electro elution, the open upper section of the tapered tube can be sealed and the desired substance is withdrawn through the cap and dialysis membrane which encloses the lower end of the tube. An auxiliary funnel can be affixed to the open upper end of the tube to increase the capacity of the system.

REFERENCES:
patent: 3579433 (1971-05-01), Dahlgren
patent: 3640813 (1972-02-01), Nerenberg
patent: 3791950 (1974-02-01), Allington
patent: 4545888 (1985-10-01), Walsh
patent: 4552640 (1985-11-01), Kartenbeck
patent: 4608147 (1986-08-01), Clad
patent: 4699706 (1987-10-01), Burd et al.
Lewis et al., "Preparative Methods for Disk Electrophoresis With Special Reference to the Isolation of Pituitary Harmones", Anal. Biochemistry 6(1963), pp. 303-315.

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