Elution and identification of T cell epitopes from viable cells

Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Cancer cell or component thereof

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514 21, 530344, 435 701, A61K 3400, A61K 3900, C07K 500, C12P 2102

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059895659

ABSTRACT:
Methods are provided for eluting peptides that are bound to major histocompatibility complex ("MHC") molecules expressed on the cell surfaces of viable cells that have at least one MHC-peptide complex on the surfaces of the cells, the method comprising incubating the cells in the presence of peptide elution buffer, preferably comprising iso-osmotic, citrate-phosphate buffer at a pH of approximately 3.3, for between about 15 seconds and one minute. Using these methods a naturally processed melanoma peptide recognized by CD8.sup.+ cytotoxic T lymphocytes has been identified.

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