Element for electrophoresis

Details Element for electrophoresis Element for electrophoresis

1986-12-29

1988-04-12

Kaplan, G. L.

Chemistry: electrical and wave energy

Apparatus

Coating, forming or etching by sputtering

428448, 4284777, C07K 314

Patent

active

047372591

ABSTRACT:
An element for electrophoresis suitably employable for electrophoresis of biopolymers such as proteins, as well as for the determination of the base sequence of DNA, RNA, their fragments, and their derivatives, which comprises a plastic support, a nonconductive metal oxide layer, and an electrophoresis medium layer comprising an aqueous polyacrylamide gel formed by crosslinking polymerization of an acrylamide compound and a crosslinking agent in the presence of water, which are superposed in this order. The electrophoresis medium layer may contain a water-soluble polymer and agarose. The medium layer may contain a modifier such as an anionic surfactant, formamide or urea.

REFERENCES:
patent: 3875044 (1975-04-01), Renn et al.
patent: 3922432 (1975-11-01), Renn
patent: 3960499 (1976-06-01), White
patent: 4415428 (1983-11-01), Nochumson et al.
patent: 4548869 (1985-10-01), Ogawa et al.
patent: 4548870 (1985-10-01), Ogawa et al.
patent: 4579783 (1986-04-01), Ogawa et al.
patent: 4600641 (1986-07-01), Ogawa et al.
patent: 4657656 (1987-04-01), Ogawa

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