Radiant energy – Ionic separation or analysis – With sample supply means
Patent
1993-11-04
1996-04-02
Beyer, James
Radiant energy
Ionic separation or analysis
With sample supply means
250281, 250282, H01J 4904
Patent
active
055043275
ABSTRACT:
An improved electrospray ionization (ESI) system source and method is presented including an electrospray ionization source for introducing an ionized molecular sample into a mass spectrometer for analysis. The ESI source is constructed in the configuration of a probe that makes use of a standard 0.5 inch (13 mm) vacuum lock commonly found on conventional mass spectrometers. The ESI probe comprises a desolvation tube, a voltage source for applying a voltage to the desolvation tube, a resistance coil for heating the desolvation tube, a sensor for measuring the temperature of the desolvation tube, a skimmer positioned downstream of the desolvation tube for directing the ions to the lens stack of the mass spectrometer, a voltage source for applying a voltage to the skimmer, a spacer lens positioned upstream of the skimmer for focusing the ions prior to their entering the skimmer, and an evacuable dielectric encasement for housing the components of the probe assembly. This novel ESI source makes available a wealth of new analytical methods and applications that heretofore were unknown to the field, including novel data interpretations of ESI results and the ability to determine the process by which protein structures and functions may be modified by the attachment of small molecules to the protein surface, particularly crown ethers.
REFERENCES:
patent: 3840742 (1974-10-01), Ball
patent: 4104275 (1978-08-01), Kauer
patent: 4209696 (1980-06-01), Fite
patent: 4271425 (1981-06-01), Wong
patent: 4367072 (1983-01-01), Vogtle et al.
patent: 4436923 (1984-03-01), Pacey et al.
patent: 4531056 (1985-07-01), Labowsky et al.
patent: 4542293 (1985-09-01), Fenn et al.
patent: 4838274 (1989-06-01), Schweighardt et al.
patent: 4842701 (1989-06-01), Smith et al.
patent: 4885076 (1989-12-01), Smith et al.
patent: 4935624 (1990-06-01), Henion et al.
patent: 4977320 (1990-12-01), Chowdhury et al.
patent: 4999493 (1991-03-01), Allen et al.
patent: 5015845 (1991-05-01), Allen et al.
patent: 5068098 (1991-11-01), Schweighardt et al.
patent: 5071581 (1991-12-01), Cipriano
patent: 5076097 (1991-12-01), Zarrinn et al.
patent: 5115131 (1992-05-01), Jorgenson et al.
patent: 5122670 (1992-06-01), Mylchreest et al.
patent: 5136161 (1992-08-01), Logan
patent: 5157260 (1992-10-01), Mylchreest et al.
patent: 5170053 (1992-12-01), Hall et al.
patent: 5171990 (1992-12-01), Mylchreest et al.
patent: 5196348 (1993-03-01), Schweighardt et al.
patent: 5223226 (1993-06-01), Wittmer et al.
patent: 5245186 (1993-09-01), Chait et al.
Papac, D. I., Schey, K. L., Knapp, D. R. "Combination Electrospray-Liquid Secondary Ion Mass Spectrometry Ion Source" Anal. Chem. 1991, 63, 1658-1660.
Chowdhury, S. K., Katta, V., Chait, B. T., "An Electrospray-ionization Mass Spectrometer with New Features" 1990.
Odell, B., and Bowyer, J. R. "Solubilisation of Ferricytochrome C in Methanol Using a Crown Ether Absorption, Circular Dichroism and EPR Spectral Properties", Biochem. Biophys. Res. Commun. 1985, 127 #3, 828-835.
Odell, B., and Earlam, G., "Dissolution of Proteins in Organic Solvents using Macrocyclic Polyethers: Association Constants of a Cytochrome C- 1,2-.sup.14 C.sub.2 !-18-Crown-6 Complex in Methanol", J. Chem. Soc., Chem. Commun., 1985, 359-361.
Chowdhury, S. K., Katta V., Chait, B. T., "Probing Conformational Changes in Proteins by Mass Spectrometry", J. Am. Chem. Soc., 1990, 112, 9012-9013.
Bowler. B. E., May, K., Zaragoza, T., York, P., Dong, A., Caughey, W. S., "Destabilizing Effects of Replacing a Surface Lysine of Cytochrome C with Aromatic Amino Acids: Implications for the Denatured State", Biochemistry 1993, 32, 183-190.
Shortle, D., Chan. H. S., Dill, K. A., "Modeling the Effects of Mutations on the Denatured States of Proteins", Protein Science, 1992, 1, 201-215.
Mirza, U. A., Cohen S. L., Chait, B. T., "Heat-Induced Conformational Changes in Proteins Studied by Electrospray Ionization Mass Spectrometry", Anal. Chem., 1993, 65, 1-6.
Kruger Terry L.
Sproch Norman
Beyer James
hv ops, Inc. (h-nu)
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