Electroporation of prokaryotic cells

Details Electroporation of prokaryotic cells Electroporation of prokaryotic cells

1990-03-14

1993-02-16

Niebling, John

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

4352523, 4351723, 435173, 935 52, B01D 5702

Patent

active

051868008

ABSTRACT:
Electroporation of prokaryotic cells is achieved by the application of high-intensity electric fields of short duration to reversibly permeabilize the cell walls. Preferably, the field strength of the applied will be at least about 5 kV/cm and the duration of the exposure will be in the range from 2 to 20 msec. Macromolecules may be introduced to the cells or obtained from the cells during this period of cell wall permeability. Most commonly, the method will be utilized for transformation of the cells with nucleic acids. Very high transformation efficiencies in the range from 10.sup.9 to 10.sup.10 cells/.mu.g and transformation frequencies approaching 80% and above have been achieved.

REFERENCES:
patent: 4081340 (1978-03-01), Zimmermann et al.
patent: 4849355 (1989-07-01), Wong
Powell et al., "A Simple and Rapid Method for Genetic Transformation of Lactic Streptocci by Electroporation," Appl. Environ. Microbiol., vol. 54, No. 3 (Mar., 1988).
BioRad, "Gene Pulser.TM. Transfection Apparatus Operating Instructions and Applications Guide" (Jan., 1987).
Gene Pulser.TM. Transfection . . . Guide, Bio-Rad Labs. pp. 1-29.
Chassy et al. (1987) FEMS Microbiol. Lett 44:173-177.
Powell et al. (1988) Appl. Environ. Microbiol. 54:655-660.
Harlander (1987) Streptococcal Genetics, pp. 229-233.
Potter et al. (1984) Proc. Natl. Acad. Sci. USA 81:7161-7165.
Shivarova et al. (1983) Zeitschrift Allge. Mikro. 23:595-599.
Miller et al. (1988) Proc. Natl. Acad. Sci. USA 85:856-860.
Dower (1987) Molecular Biology Reports 1:5.
MacNeil (1987) FEMS Microbiol. Lett 42:239-244.
Davis et al. (1968) Microbiology, Harper & Row pp. 34 and 878.
Alberts et al. (1983) Molecular Biology of the Cell, p. 923.

Affiliated with

Also associated with

Rating

Electroporation of prokaryotic cells does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Electroporation of prokaryotic cells, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Electroporation of prokaryotic cells will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2146101