Electroporation of prokaryotic cells

Details Electroporation of prokaryotic cells Electroporation of prokaryotic cells

1988-04-18

1990-03-20

Niebling, John F.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435173, 4352523, 435320, 935 52, 935 55, 935 79, 935 84, C12N 1500

Patent

active

049101404

ABSTRACT:
Electroporation of prokaryotic cells is achieved by the application of high-intensity electric fields of short duration to reversibly permeabilize the cell walls. Preferably, the field strength of the applied will be at least about 5 kV/cm and the duration of the exposure will be in the range from 2 to 20 msec. Macromolecules may be introduced to the cells or obtained from the cells during this period of cell wall permeability. Most commonly, the method will be utilized for transformation of the cells with nucleic acids. Very high transformation efficiencies in the range from 10.sup.9 to 10.sup.10 cells/.mu.g and transformation frequencies approaching 80% and above have been achieved.

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