Electroporation-mediated molecular transfer in intact plants

Details Electroporation-mediated molecular transfer in intact plants Electroporation-mediated molecular transfer in intact plants

1995-08-22

1999-01-12

Robinson, Douglas W.

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

The polynucleotide alters fat, fatty oil, ester-type wax, or...

4351721, 4351723, 4351736, 435419, C12N 1529, C12N 1582, C12N 1500, A01H 400

Patent

active

058593276

ABSTRACT:
A method for producing a genetically modified plant by introducing a polynucleotide to an intact plant or plant cell(s) via electroporation, in the absence of cell wall-degrading enzymes. Genetically engineered plants produced by the method of the invention are also provided. The invention also provides a method for producing a polypeptide in an intact plant cell, including plant tissue or a whole plant by introducing a biologically active polypeptide directly into the plant.

REFERENCES:
patent: 5236835 (1993-08-01), Mouneimne et al.
patent: 5371003 (1994-12-01), Murry et al.
patent: 5384253 (1995-01-01), Krzyzek et al.
Chowrira, et al., Electroporation-Mediated Gene Transfer into Intact Nodal Meristems In Planta, Molecular Biotechnology, vol. 3, No. 1, pp. 17-23, Feb. 1995.
Songstad, et al., Transient expression of GUS and anthocyanin constructs in intact maize immature embryos following electrporation, Plant Cell, Tissue and Organ Culture, 33: pp. 195-201, 1993.
Vani Akella and Paul F. Lurquin, Expression in Cowpea seedlings of chimeric transgenes after electroporation into seed-derived embryos, Plant Cell Reports, 12: pp. 110-117, 1993.
Senaratna, et al., Direct DNA uptake during the imbibition of dry cells, Plant Science, 79: pp. 223-228, 1991.
Dekeyser, et al., Transient Gene Expression in Intact and Organized Rice Tissues, The Plant cell, vol. 2, pp. 591-602, Jul. 1990.
Xie et al 1990 Biophys. J. 58:897-903.

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