Electroporation device and method of use

Details Electroporation device and method of use Electroporation device and method of use

1990-06-01

1999-12-14

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

435284, 435285, 435297, 435817, 2041801, 204299R, C12N 1300, C12M 300, C12M 304, C12M 122, C25B 700, B01D 6142

Patent

active

060016175

ABSTRACT:
A method and apparatus for cell culture wherein cultured cells adhere and divide, in a monolayer, on an optically transparent planar electrode in the culture vessel. An electric current is applied between the planar electrode and a counter electrode so as to create an electric field between the electrodes which influences the characteristics of the cells growing thereon. In a preferred embodiment the planar electrode may be an optically transparent conducting layer coated onto the culture vessel bottom or the culture vessel may be made of an optically transparent electrically conducting material. The apparatus is particularly useful for the introduction of different biologically important molecules into cells or for cell fusion.

REFERENCES:
patent: 4441972 (1984-04-01), Pohl
patent: 4804450 (1989-02-01), Mochizuki et al.
patent: 4832814 (1989-05-01), Root
Yaoita et al., Bioelectrochemistry and Bioenergetics 20, pp. 169-177, 1988.
Haacke, Ann. Rev. Mater. Sci., 7, pp. 73-93, 1977.
Hofmann et al. (1986) IEEE Eng. Med. Biol, Dec. pp. 6-23.

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