Electroporation cuvette with spatially variable electric field

Details Electroporation cuvette with spatially variable electric field Electroporation cuvette with spatially variable electric field

2009-02-05

2011-10-25

Nguyen, Bao Thuy L (Department: 1641)

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Cell membrane or cell surface is target

C435S449000, C435S450000, C435S461000, C435S470000, C435S285200, C435S288100

Reexamination Certificate

active

08043838

ABSTRACT:
An electroporation cuvette is constructed with electroporation electrodes arranged in non-parallel relation to form a gap whose width varies with the location within the cuvette, plus a pair of positioning electrodes that are arranged to cause electrophoretic migration of biological cells within the cuvette according to cell size. Once the cells, suspended in a solution of the impregnant, are distributed in the cuvette by the positioning electrodes, electric field pulses are generated by the non-parallel electroporation electrodes. Because of their distribution in the cuvette, the various cells will experience voltage differentials across their widths that approach uniformity regardless of cell diameter, since the larger cells will be positioned at locations where the gap between the electrodes is greater and the smaller cells at locations where the gap is relatively small while the voltage drop across the entire gap is uniform along the length of the cell. The voltage differential across the width of the cell is thus roughly paired with the cell diameter, and this reduces the disparity in voltage differential that cells of different sizes would otherwise experience with parallel electrodes.

REFERENCES:
patent: 4699881 (1987-10-01), Matschke
patent: 7678564 (2010-03-01), Muller-Hartmann et al.
patent: 2003/0022365 (2003-01-01), Marotski
patent: 2003/0124713 (2003-07-01), Ragsdale
patent: 2005/0282265 (2005-12-01), Vozza-Brown et al.
patent: 2006/0281182 (2006-12-01), Vozza-Brown et al.

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