Electroporation and electrophoresis system and method for...

Surgery – Means for introducing or removing material from body for... – Infrared – visible light – ultraviolet – x-ray or electrical...

Reexamination Certificate

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Reexamination Certificate

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07668592

ABSTRACT:
The electroporation system and method combine pulses having different characteristics for delivering molecules to cells in vivo. The pulses include a high-intensity pulse for inducing electroporation and a low-intensity pulse to induce electrophoretic molecule movement within an interstitial space, molecule adherence to a cell membrane, and electrophoretic movement of the molecule through the permeabilized membrane. The use of a high-intensity and a low-intensity pulse achieves improved delivery; reduction of intensity and/or duration of pulses for inducing electroporation; and decreased muscle stimulation, tissue damage, and patient discomfort.

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Joshi and Shoenbach, “Electroporation dynamics in biological cells subjected to ultrafast electrical pulses: A numerical simulation study”, Jul. 2000, The American Physical Society, vol. 62, No. 1, pp. 1025-1033.
Heller et al., FEBS Lett. 389, 225-228, In vivo gene electroinjection and expression in rat liver.

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