Electropipettor and compensation means for electrophoretic bias

Details Electropipettor and compensation means for electrophoretic bias Electropipettor and compensation means for electrophoretic bias

1996-06-28

1998-07-14

Valentine, Donald R.

Chemistry: electrical and wave energy

Apparatus

Electrophoretic or electro-osmotic apparatus

204451, 204453, 204454, 204601, G01N 2726, G01N 27447

Patent

active

057798680

ABSTRACT:
The present invention provides for techniques for transporting materials using electrokinetic forces through the channels of a microfluidic system. The materials are transported in slug regions of high ionic concentration, next to buffer material regions of high ionic concentration, which are separated by buffer material regions of low ionic concentration. Such arrangements allow the materials to remain localized for the transport transit time to avoid mixing of the materials. Using these techniques, an electropipettor which is compatible with the microfluidic system is created so that materials can be easily introduced into the microfluidic system. The present invention also compensates for electrophoretic bias as materials are transported through the channels of the microfluidic system by splitting a channel into portions with positive and negative surface charges and a third electrode between the two portions, or by diffusion of the electrophoresing materials after transport along a channel.

REFERENCES:
patent: 4908112 (1990-03-01), Pace
patent: 4963498 (1990-10-01), Hillman et al.
patent: 5126022 (1992-06-01), Soane et al.
patent: 5140161 (1992-08-01), Hillman et al.
patent: 5144139 (1992-09-01), Hillman et al.
patent: 5164598 (1992-11-01), Hillman et al.
patent: 5188963 (1993-02-01), Stapleton
patent: 5192405 (1993-03-01), Petersen et al.
patent: 5302264 (1994-04-01), Welch et al.
patent: 5304487 (1994-04-01), Wilding et al.
patent: 5486335 (1996-01-01), Wilding et al.
patent: 5498392 (1996-03-01), Wilding et al.
patent: 5560811 (1996-10-01), Briggs et al.
patent: 5571410 (1996-11-01), Swedberg et al.
patent: 5593838 (1997-01-01), Zanzucchi et al.
patent: 5605662 (1997-02-01), Heller et al.
patent: 5630925 (1997-05-01), Pentoney, Jr. et al.
Dasgupta, Purnendu K. et al., "Electroosmosis: A Reliable Fluid Propulsion System for Flow Injection Analysis", Anal. Chem., 1994, 66, 1792-1798.
Linhares, Michael C. et al., "Use of an On-Column Fracture in Capillary Zone Electrophoresis for Sample Introduction", Anal. Chem., 1991, 63, 2076-2078.
S. Jacobson et al., "Effects of injection schemes and column geometry on the performance of microchip electrophoresis devices," Anal. Chem. 66:1107-1113.
S. Jacobson et al., "High-speed separations on a microchip," Anal. Chem. 66:1114-1118 (1994).
S. Jacobson et al., "Fused quartz substrates for microchip electrophoresis," Anal. Chem. 67:2059-2063 (1995).
S. Jacobson et al., "Open channel electrochromatography on a microchip," Anal. Chem. 66:2369-2373 (1994).
S. Jacobson et al., "Precolumn reactions with electrophoretic analysis integrated on a microchip," Anal. Chem. 66:4127-4132 (1994).
S. Jacobson et al., "Microchip electrophoresis with sample stacking," Electrophoresis 16:481-486 (1995).
A. Manz et al., "Electroosmotic pumping and electrophoretic separations for miniaturized chemical analysis systems," J. Micromech. Microeng. 4:257-265 (1994).
J. Ramsey et al., "Microfabricated chemical measurement systems," Nature Medicine 1(10):1093 (Oct., 1995).
K. Seller et al., "Electroosmotic pumping and valveless control of fluid flow within a manifold of capillaries on a glass chip," Anal. Chem. 66:3485-3491 (1994).
Bao et al., "Ultramicro enzyme assays in a capillary electrophoretic system", J. Chromatog., 608: 212-224 (1992).
Effenhauser, et al., "Glass Chips for High-Speed Capillary Electrophoresis Separations with Submicrometer Plate Heights", Anal. Chem., 1993, 65, 2637-2642.
Fan et al., "Micromachining of Capillary Electrophoresis Injectors and Separators on Glass Chips and Evaluation of Flow at Capillary Intersections", Anal. Chem., 66, 177-184 (1994).
Harmon et al., "Mathematical Treatment of Electrophoretically Mediated Microanalysis", Anal. Chem., 65, 2655-2662, 1993.
Harmon, et al., "Selectively in Electrophoretically Mediated Microanalysis by Control of Product Detection Time", Anal. Chem., 66, 3797-3805, 1994.
Harrison, et al., "Capillary Electrophoresis and Sample Injection Systems Integrated on a Planar Glass Chip", Anal. Chem., 64, 1926-1932, 1992.
Harrison et al., "Micromachining a Miniaturized Capillary Electrophoresis-Based Chemical Analysis System on a Chip", Science, vol. 261, 895-897, (1993).
Manz et al., "Planar chips technology for miniaturization and integration of separation techniques into monitoring systems", J. Chromat., 593, 253-258, (1992).
Manz et al., "Miniaturized Total Chemical Analysis Systems: a Novel Concept for Chemical Sensing", Sensors & Actuators, B1, 244-248, (1990).
Manz et al., "Micromachining of monocrystalline silicon and glass for chemical analysis systems, A look into next century's technology or just a fashionable craze?", Trends in Anal. Chem. vol. 10, No. 5, 1991, 144-149.
Seiler, et al., "Planar Glass Chips for Capillary Electrophoresis: Repetitive Sample Injection, Quantitation, and Separation Efficiency", Anal. Chem., 1993, 65, 1481-1488.

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