Chemistry: electrical and wave energy – Processes and products – Electrophoresis or electro-osmosis processes and electrolyte...
Patent
1998-03-20
2000-06-27
Warden, Jill
Chemistry: electrical and wave energy
Processes and products
Electrophoresis or electro-osmosis processes and electrolyte...
204450, 204600, 204601, 422102, G01N 2726, G01N 27447
Patent
active
060802952
ABSTRACT:
The present invention provides for techniques for transporting materials using electrokinetic forces through the channels of a microfluidic system. The subject materials are transported in regions of high ionic concentration, next to spacer material regions of high ionic concentration, which are separated by spacer material regions of low ionic concentration. Such arrangements allow the materials to remain localized for the transport transit time to avoid mixing of the materials. Using these techniques, an electropipettor which is compatible with the microfluidic system is created so that materials can be easily introduced into the microfluidic system. The present invention also compensates for electrophoretic bias as materials are transported through the channels of the microfluidic system by splitting a channel into portions with positive and negative surface charges and a third electrode between the two portions, or by diffusion of the electrophoresing materials after transport along a channel.
REFERENCES:
patent: 4908112 (1990-03-01), Pace
patent: 4963498 (1990-10-01), Hillman et al.
patent: 5089099 (1992-02-01), Chine et al.
patent: 5116471 (1992-05-01), Chien et al.
patent: 5126022 (1992-06-01), Soane et al.
patent: 5140161 (1992-08-01), Hillman et al.
patent: 5144139 (1992-09-01), Hillman et al.
patent: 5164598 (1992-11-01), Hillman et al.
patent: 5188963 (1993-02-01), Stapleton
patent: 5192405 (1993-03-01), Peterson et al.
patent: 5270183 (1993-12-01), Corbett et al.
patent: 5282942 (1994-02-01), Herrick et al.
patent: 5302264 (1994-04-01), Welch et al.
patent: 5304487 (1994-04-01), Wilding et al.
patent: 5358612 (1994-10-01), Dasgupta et al.
patent: 5415747 (1995-05-01), Holloway
patent: 5486335 (1996-01-01), Wilding et al.
patent: 5498392 (1996-03-01), Wilding et al.
patent: 5536382 (1996-07-01), Sunzeri
patent: 5560811 (1996-10-01), Briggs et al.
patent: 5571410 (1996-11-01), Swedberg et al.
patent: 5593838 (1997-01-01), Zanzucchi et al.
patent: 5605662 (1997-02-01), Heller et al.
patent: 5630925 (1997-05-01), Pentoney, Jr. et al.
patent: 5856174 (1999-01-01), Lipshutz et al.
Jacobson et al., "Effects of Injection Schemes and Column Geometry on the Performance of Microchip Electrophoresis Devices," Anal. Chem. (1994) 66:1107-1113.
Jacobson et al., "High-Speed Separations on a Microchip," Anal. Chem. (1994) 66:1114-1118.
Jacobson et al., "Fused Quartz Substrates for Microchip Electrophoresis," Anal. Chem. (1995) 67:2059-2063.
Jacobson et al., "Open Channel Electrochromatography on a Microchip," Anal. Chem. (1994) 66:2369-2373.
Jacobson et al., "Precolumn Reactions with Electrophoretic Analysis Integrated on a Microchip," Anal. Chem. (1994) 66:4127-4124.
Jacobson et al., "Microchip electrophoresis with sample stacking," Electrophoresis (1995) 16:481-486.
Manz et al., "Electroosmotic pumping and electrophoretic separations for miniaturized chemical analysis systems," J. Micromech. Microeng. (1994) 4:257-265.
Ramsey et al., "Microfabricated chemical measurement systems," Nature Medicine (1995) 1:1093-1096.
Seiler et al., "Electroosmotic Pumping and Valveless Control of Fluid Flow within a Manifold of Capillaries on a Glass Chip," Anal. Chem. (1994) 66:3485-3491.
Dasgupta et al., "Electroosmosis: A Reliable Fluid Propulsion System for Flow Injection Analysis," Anal. Chem. (1994) 66:1792-1798.
Linhares et al., "Use of an On-Column Fracture in Capillary Zone Electrophoresis for Sample Introduction," Anal. Chem. (1991) 63:2076-2078.
Bao et al., "Ultramicro enzyme assays in a capillary electrophoretic system," J. Chromatography (1992) 608:217-224.
Effenhauser et al., "Glass Chips for High-Speed Capillary Electrophoresis Separations with Submicrometer Plate Heights," Anal. Chem. (1993) 65:2637-2642.
Fan et al., "Micromachining of Capillary Electrophoresis Injectors and Separators on Glass Chips and Evaluation of Flow at Capillary Intersections," Anal. Chem. (1994) 66:177-184.
Harmon et al., "Mathematical Treatment of Electrophoretically Mediated Microanalysis," Anal. Chem. (1993) 65:2655-2662.
Harmon et al., "Selectivity in Electrophoretically Mediated Microanalysis by Control of Product Detection Time," Anal. Chem. (1994) 66:3797-3805.
Harrison et al., "Capillary Electrophoresis and Sample Injection Systems Integrated on a Planar Glass Chip," Anal. Chem. (1992) 64:1926-1932.
Harrison et al., "Micromachining a Miniaturized Capillary Electrophoresis-Based Chemical Analysis System on a Chip," Science (1993) 261:895-897.
Manz et al., "Planar chips technology for miniaturization and integration of separation techniques into monitoring systems," J. Chromatography (1992) 593:253-258.
Manz et al., "Miniaturized Total Chemical Analysis Systems: a Novel Concept for Chemical Sensing," Sensors and Actuators (1990) B1:244-248.
Manz et al., "Micromachining of monocrystaline silicon and glass for chemical analysis systems," Trends in Analytical Chemistry (1991) 10:144-149.
Seiler et al., "Planar Glass Chips for Capillary Electrophoresis: Repetitive Sample Injection, Quantitation, and Separation Efficiency," Anal. Chem. (1993) 65:1481-1488.
Sandoval et al., "Method for the Accelerated Measurement of Electroosmosis in Chemically Modified Tubes for Capillary Electrophoresis," Anal. Chem. (1996) 68:2771-2775.
Svendsen et al., "Separation of Proteins Using Ampholine Carrier Ampholytes as Buffer and Spacer Ions in an Isotachophoresis System," Science Tools, The KLB Instrument Journal (1970) 17:13-17.
Kjellin et al., "Isotachophoresis of CSF Proteins in Gel Tubes Especially Gammaglobulins," J. Neurol. (1979) 221:225-233.
Kopwillem et al., "Serum Protein Fractionation by Isotachophoresis Using Amino Acid Spacers," J. Chromatography (1976) 118:35-46.
Holloway et al., "The analysis of amino acids and peptides by isotachophoresis," Electrophoresis (1981) 2:127-134.
Knapp Michael R.
Parce J. Wallace
Caliper Technologies Corporation
Starsiak Jr. John S.
Warden Jill
LandOfFree
Electropipettor and compensation means for electrophoretic bias does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Electropipettor and compensation means for electrophoretic bias, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Electropipettor and compensation means for electrophoretic bias will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1781292