Electrophoretogram analytical image processing system

Details Electrophoretogram analytical image processing system Electrophoretogram analytical image processing system

1983-08-15

1986-05-27

Boudreau, Leo H.

Image analysis

Histogram processing

For setting a threshold

358 93, 358107, 382 48, G06K 900

Patent

active

045920893

ABSTRACT:
An analytical image processing system is used to analyze spots on color electrophoretograms. In one embodiment, the boundary of a spot chosen by the user is uniquely defined using polar cross-sections and is illustrated on a user interactive display. In such manner, the user is provided with visual confirmation of the shape of the spot used as a basis for generating output data relating to the spot analysis. The output data includes information not only about the two-dimensional area of the spot but also the intensity thereof to provide a useful measure of the quantity of protein present in the spot.

REFERENCES:
patent: 4167729 (1979-09-01), Christenson et al.
patent: 4389670 (1983-06-01), Davidson et al.
Taylor et al., "Design and Implementation of a Prototype Human Protein Index", Clinical Chemistry, vol. 28, No. 4, (1982).
Lester et al., "Computer-Assisted Analysis of Two-Dimensional Electrophoresis of Human Lymphoid Cells", Clinical Chemistry, vol. 26, No. 10, (1980).
Bossinger et al., "Quantitative Analysis of Two-Dimensional Electrophoretograms", Journal of Biological Chemistry, vol. 254, No. 16, (1979).
Kronberg et al., "Photometric Evaluation of Slab Gels", Electrophoresis, pp. 27-32, (1980).
Adams et al., "A Unique Silver Staining Procedure for Color Characterization of Polypeptides", in: Electrophoresis, (1981), Allen et al. (Eds.), Walter deGruyter and Company, pp. 155-167.
Vincent et al., "Multispectral Digital Image Analysis of Color Two-Dimensional Electrophoretograms", presented at Electrophoresis '81 Symposium, Apr. 8, 1981.

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