Electrophoretic system including means for replacing separation

Details Electrophoretic system including means for replacing separation Electrophoretic system including means for replacing separation

1995-08-23

1997-06-03

Gorgos, Kathryn L.

Chemistry: electrical and wave energy

Apparatus

Electrophoretic or electro-osmotic apparatus

204455, 204453, 204604, G01N 2726

Patent

active

056350503

ABSTRACT:
A gel pump operated by a stepper motor may be used to deliver fresh gel to one or more capillaries through one or more manifolds and valves. By controlling the valves, fresh gel delivered by the pump will replace the old gel in the capillaries for an automated gel replacement system. In a different setting of the valves, the manifolds may be purged of the old gel prior to delivery of fresh gel to the capillaries. The gel delivery system may also be combined with an electrophoresis system so that sealing connection capable of withstanding high pressure adequate for gel injection need not be frequently broken when the gel is to be replaced. Manifold/reflector assembly is advantageously used in the system that facilitates electrical circuit for electrophoresis and for gel replacement.

REFERENCES:
patent: 5037523 (1991-08-01), Weinberger et al.
patent: 5332481 (1994-07-01), Guttman
patent: 5427729 (1995-06-01), Dubrow
"Pressure Refilled Polyacrylamide Columns for the Separation of Oligonucleotides by Capillary Electrophoresis," by Jan Sudor, Electrophoresis, 1991, 12, 1056-1058.

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