Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving transferase
Patent
1982-05-17
1983-11-15
Shapiro, Lionel M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving transferase
435 26, 435190, 435194, 435197, 435814, C12Q 150, C12Q 132, C12N 904, C12N 912
Patent
active
044156553
ABSTRACT:
A method for separating isoenzymes is disclosed. The method involves polyacrylamide gel electrophoresis in a buffer solution of a salt of 2-amino-2-methyl-1,3-propanediol at a pH of about 6.4 to 7.3 and an electrolyte buffer of 2-amino-2-methyl-1,3-propanediol taurine at a pH of about 8.0 to 10.0. After separation of the isoenzymes, the amount of individual isoenzymes present is determined.
REFERENCES:
patent: 4338396 (1982-07-01), Kiyasu
J. Biochem., 71(3):543, (1972), H. Tamura & N. Ui, "A New Buffer System for Disc Electrophoresis . . . Proteins".
Analytical Biochem., 78:459, (1977), M. Wyckoff et al., "Polyacrylamide Gel Electrophoresis . . . Procedure".
Anal. Biochem., 20:246, (1967), A. A. Dietz et al., "Separation and Quantitation of Lactic . . . Electrophoresis".
Clin. Chim. Acta., 21:151, (1968), T. D. Trainer et al., "A Rapid Method for the Analysis of Creatine . . . Isoenzymes".
"Methods of Protein Separations," vol. 2, 1976, Table of Contents of Chapters 2 to 4.
Clin. Biochem., 7:29, (1974), S. B. Rosalki, "Standardization of Isoenzyme Assays with Special Reference . . . Electrophoresis".
Am. J. of Clin. Path., 43(3):256, (1965), J. A. Preston et al., "Rapid Electrophoretic Separation of Lactate . . . Acetate".
Anal. Biochem., 63:241, (1975), K. Ariyoshi et al., "An Apparatus for Thin Layer Vertical . . . Gel System".
J. Urology, 118:204, (1977), J. T. Gayhack et al., "Lactate Dehydrogenase Isoenzymes in Human . . . Malignancy?".
Anals of Clin. Lab. Sci., 4:456, (1974), D. J. Blomberg et al., "Isoenzymes of Creatine Kinase; Separation . . . Electrophoresis".
de Castro Aurora F.
Gupta Surendra K.
Shapiro Lionel M.
TechAmerica Group, Inc.
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