Electrophoretic resolution of single strand DNA by asymmetric fi

Chemistry: electrical and wave energy – Processes and products – Electrostatic field or electrical discharge

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204299R, 2041821, G01N 2726, G01N 27447, B01D 5702

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active

051787377

ABSTRACT:
Single stranded DNA fragments in sizes above 300 bases are separated electrophoretically in a field inversion technique in which the voltage of the reverse pulse exceeds that of the forward pulse, the duration of the forward pulse exceeds that of the reverse pulse, and the product of voltage and duration for the forward pulse exceeds that of the reverse pulse. The result is an improvement in resolution over previous field inversion techniques, and the elimination of band inversion.

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