Electrophoretic processor and methods

Details Electrophoretic processor and methods Electrophoretic processor and methods

1991-10-25

1993-04-06

Niebling, John

Chemistry: electrical and wave energy

Apparatus

Coating, forming or etching by sputtering

2041801, 204301, G01N 2726, G01N 27447

Patent

active

052000508

ABSTRACT:
An electrophoretic processor for separating proteins and other chemicals exhibiting varying electrophoretic mobilities. The preferred processor includes a rotor which turns within a stator to define a processing chamber therebetween. The rotor and stator are preferably cylindrical to provide an cylindrically annular processing chamber which will induce transverse secondary flows, preferably in the form of toroidal vortices. The transverse toroidal vortices improve heat transfer and counteract longitudinal flows which decrease separation. The processor can be provided with a process temperature stabilizer, such as a chamber surrounding the stator, through which a heat exchange fluid is passed. The processor can be used to perform a variety of processes including batch and continuous flow zone electrophoresis and batch isoelectric focusing.

REFERENCES:
patent: 4900421 (1990-02-01), Grutzner et al.
Rotofor brochure entitled A Revolutionary Device for High Resolution Preparative Electrophoresis by Bio-Rad Laboratories Chemical Division (undated).
Article entitled, The Development of Recycle Zone Electrophoresis by Cornelius F. Ivory, Department of Chemical Engineering, Washington State University, 1990.

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