Electrophoretic processing

Details Electrophoretic processing Electrophoretic processing

1993-01-15

1994-03-29

Niebling, John

Chemistry: electrical and wave energy

Apparatus

Coating, forming or etching by sputtering

204300R, 204299R, 2041801, G01N 2726, G01N 27447, B01D 5702

Patent

active

052981436

ABSTRACT:
An electrophoretic processor for separating proteins and other chemicals exhibiting varying electrophoretic mobilities. The preferred processor includes a rotor which turns within a stator to define a processing chamber therebetween. The rotor and stator are preferably cylindrical to provide an cylindrically annular processing chamber which will induce transverse secondary flows, preferably in the form of toroidal vortices. The transverse toroidal vortices improve heat transfer and counteract longitudinal flows which decrease separation. Embodiments are described which include multizone electric fields. The resulting electric fields are varied in strength to focus mobile molecules against a countervailing flow of carrier fluid. The described processors can be used to perform a variety of processes including batch and continuous flow zone electrophoresis and batch isoelectric focusing.

REFERENCES:
patent: 3563872 (1971-02-01), Huebner
patent: 3616453 (1971-10-01), Philpot
patent: 3758395 (1973-09-01), Strickler
patent: 4670119 (1987-06-01), Hurd
patent: 4732656 (1988-03-01), Hurd
patent: 4900421 (1990-02-01), Grutzner et al.
Rotofor brochure entitled A Revolutionary Device for High Resolution Preparative Electrophoresis by Bio-Rad Laboratories Chemical Division (undated).
Article entitled The Development of Recycle Zone Electrophoresis by Cornelius F. Ivory, Department of Chemical Engineering, Washington State University, 1990.

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